Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 888883..889537 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GBG22_RS04305 Protein ID WP_000244781.1
Coordinates 889130..889537 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GBG22_RS04300 Protein ID WP_000354046.1
Coordinates 888883..889149 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS04280 884971..886404 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
GBG22_RS04285 886449..886760 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
GBG22_RS04290 886924..887583 + 660 WP_000250274.1 hemolysin III family protein -
GBG22_RS04295 887660..888640 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
GBG22_RS04300 888883..889149 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GBG22_RS04305 889130..889537 + 408 WP_000244781.1 protein YgfX Toxin
GBG22_RS04310 889577..890098 - 522 WP_001055874.1 flavodoxin FldB -
GBG22_RS04315 890210..891106 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
GBG22_RS04320 891131..891841 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GBG22_RS04325 891847..893580 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T141419 WP_000244781.1 NZ_CP045206:889130-889537 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T141419 NZ_CP059959:c447145-446729 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAATGCACGCTGCGGCGTGATCGTATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTTGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCAGGCACACCTATTGGTCCAAACGATACGGCTATAGC
GGGCCATGCCATCAGCGTTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTTACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT141419 WP_000354046.1 NZ_CP045206:888883-889149 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT141419 NZ_CP059959:c447369-447142 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CAAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAACCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References