Detailed information of TA system TA141406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4691806..4692616
• Replicon: chromosome
• Accession: NZ_CP045193
• Organism: Klebsiella pneumoniae strain YML0508

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: F8E20_RS22580
• Protein ID: WP_014908042.1
• Coordinates: 4691806..4692339 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: F8E20_RS22585
• Protein ID: WP_023343051.1
• Coordinates: 4692350..4692616 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8E20_RS22575	4690637..4691758	+	1122	WP_012737335.1	cupin domain-containing protein	-
F8E20_RS22580	4691806..4692339	-	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
F8E20_RS22585	4692350..4692616	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
F8E20_RS22590	4692719..4694152	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
F8E20_RS22595	4694142..4694825	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
F8E20_RS22600	4694998..4696383	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
F8E20_RS22605	4696401..4696745	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T141406 WP_014908042.1 NZ_CP045193:c4692339-4691806 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T141406 NZ_CP059958:1743133-1743438 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT141406 WP_023343051.1 NZ_CP045193:c4692616-4692350 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT141406 NZ_CP059958:1742912-1743130 [Pectobacterium brasiliense]
ATGAAACACCGCGTTAGTGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCAGATGAAAACAGGAATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A332NV98