Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4256513..4257010 Replicon chromosome
Accession NZ_CP045190
Organism Escherichia coli strain NT1F31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GAG93_RS21630 Protein ID WP_001173343.1
Coordinates 4256513..4256686 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GAG93_RS21635 Protein ID WP_000398619.1
Coordinates 4256714..4257010 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GAG93_RS21620 4253183..4254640 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
GAG93_RS21625 4254877..4256394 + 1518 WP_001295074.1 lysine--tRNA ligase -
GAG93_RS21630 4256513..4256686 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GAG93_RS21635 4256714..4257010 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GAG93_RS21640 4257237..4257509 - 273 WP_000405647.1 N-acetyltransferase -
GAG93_RS21645 4257521..4257751 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
GAG93_RS21650 4257932..4259563 + 1632 WP_001565376.1 sensor histidine kinase -
GAG93_RS21655 4259560..4260279 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4238204..4257010 18806


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T141391 WP_001173343.1 NZ_CP045190:c4256686-4256513 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T141391 NZ_CP059957:711157-712476 [Pectobacterium brasiliense]
ATGGCTGCTCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATACGAACTGGCTCGGATTGCTGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAT
ACGGATTCCACCCAACCGTTTGATCTGCTCGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATATAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATT
TGTCCGATGCCCCGCTGGGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCA
TTGCTTTATCTGGATGATCGCTGGTGCCTGCCTCTCAATGTCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTAGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTTATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGACTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCCAAGGTCGTTGGGTTCGCCAGAGAATCGATGGAAAACATTCTGGCGGAGTTTGCCCAGACCGTT
GATACCGTCATGACGACTGTTGGAAGTCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT141391 WP_000398619.1 NZ_CP045190:c4257010-4256714 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT141391 NZ_CP059957:710888-711157 [Pectobacterium brasiliense]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGGAA
AGTGGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGTTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References