Detailed information of TA system TA141358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1241205..1242122
• Replicon: chromosome
• Accession: NZ_CP045186
• Organism: Bacillus velezensis strain FJAT-52631

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: F9286_RS06535
• Protein ID: WP_007407256.1
• Coordinates: 1241376..1242122 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F9286_RS06530
• Protein ID: WP_003154807.1
• Coordinates: 1241205..1241375 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9286_RS06495	1236438..1238060	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
F9286_RS06500	1238073..1238444	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
F9286_RS06505	1238449..1238646	+	198	WP_032874605.1	XkdX family protein	-
F9286_RS06510	1238703..1239464	+	762	WP_032874607.1	hypothetical protein	-
F9286_RS06515	1239516..1239779	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F9286_RS06520	1239793..1240056	+	264	WP_003154813.1	phage holin	-
F9286_RS06525	1240070..1240948	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
F9286_RS06530	1241205..1241375	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F9286_RS06535	1241376..1242122	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F9286_RS06540	1242227..1243225	-	999	WP_032874611.1	inorganic phosphate transporter	-
F9286_RS06545	1243238..1243855	-	618	WP_032874614.1	DUF47 domain-containing protein	-
F9286_RS06550	1244141..1245457	-	1317	WP_032874616.1	amino acid permease	-
F9286_RS06555	1245779..1246729	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1197451..1299974	102523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T141358 WP_007407256.1 NZ_CP045186:c1242122-1241376 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T141358 NZ_CP059955:c425230-424814 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAGTGCACGCTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCTGGCACACCTATTGGTCCAAACGATACGGCCATCGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT141358 WP_003154807.1 NZ_CP045186:c1241375-1241205 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT141358 NZ_CP059955:c425454-425227 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAGCCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCCGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14