Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3290046..3290666 Replicon chromosome
Accession NZ_CP045163
Organism Yersinia pestis strain SCPM-O-B-6291 (C-25)

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag GCK68_RS15310 Protein ID WP_002208622.1
Coordinates 3290463..3290666 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag GCK68_RS15305 Protein ID WP_002218472.1
Coordinates 3290046..3290414 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK68_RS15290 3288241..3288501 + 261 WP_002208617.1 type B 50S ribosomal protein L31 -
GCK68_RS15295 3288517..3288660 + 144 WP_002208618.1 type B 50S ribosomal protein L36 -
GCK68_RS15300 3289534..3289887 + 354 WP_002208619.1 hypothetical protein -
GCK68_RS15305 3290046..3290414 + 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
GCK68_RS15310 3290463..3290666 + 204 WP_002208622.1 expression modulating protein YmoA Toxin
GCK68_RS15320 3291554..3291943 + 390 WP_002208623.1 MGMT family protein -
GCK68_RS15325 3292079..3292600 - 522 WP_002208624.1 lipoprotein -
GCK68_RS15330 3292857..3293717 + 861 WP_002208625.1 acyl-CoA thioesterase II -
GCK68_RS15335 3293977..3295266 - 1290 WP_002228344.1 ammonium transporter AmtB -
GCK68_RS15340 3295306..3295644 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T141356 WP_002208622.1 NZ_CP045163:3290463-3290666 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T141356 NZ_CP059948:116835-117251 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT141356 WP_002218472.1 NZ_CP045163:3290046-3290414 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT141356 NZ_CP059948:116608-116838 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References