Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 1817236..1817882 Replicon chromosome
Accession NZ_CP045163
Organism Yersinia pestis strain SCPM-O-B-6291 (C-25)

Toxin (Protein)


Gene name hicA Uniprot ID A0A3G5L3F2
Locus tag GCK68_RS08185 Protein ID WP_002214976.1
Coordinates 1817700..1817882 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0U1R391
Locus tag GCK68_RS08180 Protein ID WP_002211129.1
Coordinates 1817236..1817655 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK68_RS08160 1812307..1813302 + 996 WP_002211125.1 substrate-binding domain-containing protein -
GCK68_RS08165 1813368..1814945 + 1578 WP_002211126.1 sugar ABC transporter ATP-binding protein -
GCK68_RS08170 1814948..1815970 + 1023 WP_002214974.1 ABC transporter permease -
GCK68_RS08175 1816019..1816978 + 960 WP_002223882.1 sugar kinase -
GCK68_RS08180 1817236..1817655 - 420 WP_002211129.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GCK68_RS08185 1817700..1817882 - 183 WP_002214976.1 type II toxin-antitoxin system HicA family toxin Toxin
GCK68_RS08190 1818089..1818871 - 783 WP_002211130.1 flagellar type III secretion system protein FliR -
GCK68_RS08195 1818874..1819143 - 270 WP_002211131.1 flagellar biosynthesis protein FliQ -
GCK68_RS08200 1819249..1819989 - 741 WP_002220494.1 flagellar type III secretion system pore protein FliP -
GCK68_RS08205 1820070..1820606 - 537 WP_002211133.1 flagellar biosynthetic protein FliO -
GCK68_RS08210 1820606..1821022 - 417 WP_002211134.1 flagellar motor switch protein FliN -
GCK68_RS08215 1821019..1822020 - 1002 WP_002211135.1 flagellar motor switch protein FliM -
GCK68_RS08220 1822026..1822496 - 471 WP_002227961.1 flagellar basal body-associated protein FliL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-131)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6783.99 Da        Isoelectric Point: 10.8877

>T141354 WP_002214976.1 NZ_CP045163:c1817882-1817700 [Yersinia pestis]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Download         Length: 183 bp

>T141354 NZ_CP059948:c98101-97943 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15549.42 Da        Isoelectric Point: 4.7731

>AT141354 WP_002211129.1 NZ_CP045163:c1817655-1817236 [Yersinia pestis]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Download         Length: 420 bp

>AT141354 NZ_CP059948:c98369-98145 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G5L3F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0U1R391

References