Detailed information of TA system TA141332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2583704..2584350
• Replicon: chromosome
• Accession: NZ_CP045158
• Organism: Yersinia pestis subsp. pestis bv. Medievalis strain SCPM-O-B-6530

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G5L3F2
• Locus tag: DJY80_RS12355
• Protein ID: WP_002214976.1
• Coordinates: 2583704..2583886 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0U1R391
• Locus tag: DJY80_RS12360
• Protein ID: WP_002211129.1
• Coordinates: 2583931..2584350 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DJY80_RS12320	2579090..2579560	+	471	WP_002227961.1	flagellar basal body-associated protein FliL	-
DJY80_RS12325	2579566..2580567	+	1002	WP_002211135.1	flagellar motor switch protein FliM	-
DJY80_RS12330	2580564..2580980	+	417	WP_002211134.1	flagellar motor switch protein FliN	-
DJY80_RS12335	2580980..2581516	+	537	WP_002211133.1	flagellar biosynthetic protein FliO	-
DJY80_RS12340	2581597..2582337	+	741	WP_002220494.1	flagellar type III secretion system pore protein FliP	-
DJY80_RS12345	2582443..2582712	+	270	WP_002211131.1	flagellar biosynthesis protein FliQ	-
DJY80_RS12350	2582715..2583497	+	783	WP_002211130.1	flagellar type III secretion system protein FliR	-
DJY80_RS12355	2583704..2583886	+	183	WP_002214976.1	type II toxin-antitoxin system HicA family toxin	Toxin
DJY80_RS12360	2583931..2584350	+	420	WP_002211129.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DJY80_RS12365	2584608..2585567	-	960	WP_002223882.1	sugar kinase	-
DJY80_RS12370	2585616..2586638	-	1023	WP_002214974.1	ABC transporter permease	-
DJY80_RS12375	2586641..2588218	-	1578	WP_002211126.1	sugar ABC transporter ATP-binding protein	-
DJY80_RS12380	2588284..2589279	-	996	WP_002211125.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6783.99 Da
• Isoelectric Point: 10.8877

>T141332 WP_002214976.1 NZ_CP045158:2583704-2583886 [Yersinia pestis subsp. pestis bv. Medievalis]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Nucleotide

• Length: 183 bp

>T141332 NZ_CP059947:1018233-1018568 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15549.42 Da
• Isoelectric Point: 4.7731

>AT141332 WP_002211129.1 NZ_CP045158:2583931-2584350 [Yersinia pestis subsp. pestis bv. Medievalis]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Nucleotide

• Length: 420 bp

>AT141332 NZ_CP059947:1017985-1018233 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3G5L3F2

Antitoxin

• AlphaFold DB: A0A0U1R391