Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1318077..1318697 Replicon chromosome
Accession NZ_CP045154
Organism Yersinia pestis strain SCPM-O-B-5935 (I-1996)

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag GCK71_RS06290 Protein ID WP_002208622.1
Coordinates 1318077..1318280 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag GCK71_RS06295 Protein ID WP_002218472.1
Coordinates 1318329..1318697 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK71_RS06260 1313099..1313437 + 339 WP_002208627.1 P-II family nitrogen regulator -
GCK71_RS06265 1313477..1314766 + 1290 WP_002228344.1 ammonium transporter AmtB -
GCK71_RS06270 1315026..1315886 - 861 WP_002208625.1 acyl-CoA thioesterase II -
GCK71_RS06275 1316143..1316664 + 522 WP_002208624.1 lipoprotein -
GCK71_RS06280 1316800..1317189 - 390 WP_002208623.1 MGMT family protein -
GCK71_RS06290 1318077..1318280 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
GCK71_RS06295 1318329..1318697 - 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
GCK71_RS06300 1318856..1319209 - 354 WP_002208619.1 hypothetical protein -
GCK71_RS06305 1320083..1320226 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
GCK71_RS06310 1320242..1320502 - 261 WP_002208617.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T141315 WP_002208622.1 NZ_CP045154:c1318280-1318077 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T141315 NZ_CP059929:1478830-1479108 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT141315 WP_002218472.1 NZ_CP045154:c1318697-1318329 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT141315 NZ_CP059929:1478567-1478827 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References