Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/ParE-CC2985 |
| Location | 52232..52766 | Replicon | plasmid pCD |
| Accession | NZ_CP045151 | ||
| Organism | Yersinia pestis strain SCPM-O-DNA-18 (I-3113) | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | Q663N8 |
| Locus tag | GCK70_RS22255 | Protein ID | WP_002213360.1 |
| Coordinates | 52467..52766 (+) | Length | 100 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | Q9ZGW3 |
| Locus tag | GCK70_RS22250 | Protein ID | WP_002224342.1 |
| Coordinates | 52232..52474 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GCK70_RS22230 | 48842..50008 | + | 1167 | WP_002220902.1 | plasmid-partitioning protein SopA | - |
| GCK70_RS22235 | 50005..50970 | + | 966 | WP_002213354.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| GCK70_RS22740 | 51130..51366 | + | 237 | WP_154020274.1 | hypothetical protein | - |
| GCK70_RS22935 | 51515..51688 | - | 174 | WP_002213357.1 | hypothetical protein | - |
| GCK70_RS22245 | 51774..52066 | + | 293 | Protein_65 | IS6 family transposase | - |
| GCK70_RS22250 | 52232..52474 | + | 243 | WP_002224342.1 | type II toxin-antitoxin system ParD family antitoxin | Antitoxin |
| GCK70_RS22255 | 52467..52766 | + | 300 | WP_002213360.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| GCK70_RS22260 | 52906..53565 | - | 660 | WP_002229754.1 | type III secretion system effector GTPase activator YopE | - |
| GCK70_RS22265 | 53759..54151 | + | 393 | WP_002213403.1 | type III secretion system chaperone SycE/YerA | - |
| GCK70_RS22270 | 54214..54858 | - | 645 | Protein_70 | IS3 family transposase | - |
| GCK70_RS22275 | 54961..56133 | + | 1173 | WP_002220893.1 | IS21 family transposase | - |
| GCK70_RS22280 | 56133..56531 | + | 399 | Protein_72 | ATP-binding protein | - |
| GCK70_RS22285 | 56908..57333 | + | 426 | WP_002213267.1 | type III secretion system chaperone SycH | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA | 1..68343 | 68343 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 100 a.a. Molecular weight: 11632.19 Da Isoelectric Point: 5.5114
>T141313 WP_002213360.1 NZ_CP045151:52467-52766 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE
Download Length: 300 bp
>T141313 NZ_CP059929:1265181-1265399 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 8865.15 Da Isoelectric Point: 6.4781
>AT141313 WP_002224342.1 NZ_CP045151:52232-52474 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV
Download Length: 243 bp
>AT141313 NZ_CP059929:1264781-1265155 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q663N8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E1NQY6 |