Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 2384231..2384877 Replicon chromosome
Accession NZ_CP045149
Organism Yersinia pestis strain SCPM-O-DNA-18 (I-3113)

Toxin (Protein)


Gene name hicA Uniprot ID A0A3G5L3F2
Locus tag GCK70_RS11430 Protein ID WP_002214976.1
Coordinates 2384231..2384413 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0U1R391
Locus tag GCK70_RS11435 Protein ID WP_002211129.1
Coordinates 2384458..2384877 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK70_RS11395 2379617..2380087 + 471 WP_002227961.1 flagellar basal body-associated protein FliL -
GCK70_RS11400 2380093..2381094 + 1002 WP_002211135.1 flagellar motor switch protein FliM -
GCK70_RS11405 2381091..2381507 + 417 WP_002211134.1 flagellar motor switch protein FliN -
GCK70_RS11410 2381507..2382043 + 537 WP_002211133.1 flagellar biosynthetic protein FliO -
GCK70_RS11415 2382124..2382864 + 741 WP_002220494.1 flagellar type III secretion system pore protein FliP -
GCK70_RS11420 2382970..2383239 + 270 WP_002211131.1 flagellar biosynthesis protein FliQ -
GCK70_RS11425 2383242..2384024 + 783 WP_002211130.1 flagellar type III secretion system protein FliR -
GCK70_RS11430 2384231..2384413 + 183 WP_002214976.1 type II toxin-antitoxin system HicA family toxin Toxin
GCK70_RS11435 2384458..2384877 + 420 WP_002211129.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GCK70_RS11440 2385135..2386094 - 960 WP_002211128.1 sugar kinase -
GCK70_RS11445 2386143..2387165 - 1023 WP_002214974.1 ABC transporter permease -
GCK70_RS11450 2387168..2388745 - 1578 WP_002211126.1 sugar ABC transporter ATP-binding protein -
GCK70_RS11455 2388811..2389806 - 996 WP_002211125.1 substrate-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-131)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6783.99 Da        Isoelectric Point: 10.8877

>T141302 WP_002214976.1 NZ_CP045149:2384231-2384413 [Yersinia pestis]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Download         Length: 183 bp

>T141302 NZ_CP059918:c37957-37775 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15549.42 Da        Isoelectric Point: 4.7731

>AT141302 WP_002211129.1 NZ_CP045149:2384458-2384877 [Yersinia pestis]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Download         Length: 420 bp

>AT141302 NZ_CP059918:c38151-37954 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G5L3F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0U1R391

References