Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /COG5642-COG5654
Location 1918763..1919729 Replicon chromosome
Accession NZ_CP045149
Organism Yersinia pestis strain SCPM-O-DNA-18 (I-3113)

Toxin (Protein)


Gene name - Uniprot ID A0A0E8XJV6
Locus tag GCK70_RS09085 Protein ID WP_002210981.1
Coordinates 1918763..1919221 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q66A69
Locus tag GCK70_RS09090 Protein ID WP_002210982.1
Coordinates 1919283..1919729 (-) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK70_RS09055 1914577..1915242 + 666 WP_002210975.1 phage shock protein PspA -
GCK70_RS09060 1915404..1915631 + 228 WP_002210976.1 envelope stress response membrane protein PspB -
GCK70_RS09065 1915631..1915990 + 360 WP_002210977.1 envelope stress response membrane protein PspC -
GCK70_RS09070 1916075..1916317 + 243 WP_002216375.1 phage shock protein PspD -
GCK70_RS09075 1916298..1917695 + 1398 WP_002210979.1 YcjX family protein -
GCK70_RS09080 1917692..1918753 + 1062 WP_002210980.1 YcjF family protein -
GCK70_RS09085 1918763..1919221 - 459 WP_002210981.1 RES family NAD+ phosphorylase Toxin
GCK70_RS09090 1919283..1919729 - 447 WP_002210982.1 DUF2384 domain-containing protein Antitoxin
GCK70_RS09095 1920065..1921642 + 1578 WP_002210983.1 transcriptional regulator TyrR -
GCK70_RS09100 1921789..1922247 - 459 WP_002213775.1 IS200/IS605-like element IS1541B family transposase -
GCK70_RS09105 1922500..1923003 - 504 WP_002210984.1 thiol peroxidase -
GCK70_RS09110 1923166..1924140 + 975 WP_002210985.1 L-Ala-D/L-Glu epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1921789..1922247 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(2-147)

Antitoxin

Antitoxin Xre

(15-147)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 16994.38 Da        Isoelectric Point: 4.8732

>T141301 WP_002210981.1 NZ_CP045149:c1919221-1918763 [Yersinia pestis]
MTLYRIVMRRYLASAWTGYGAEIYGGRWNHKGHAAIYLASSISLAMLETLAHIQDSSTLSEFELFQIEISDSSIMLLQPQ
DWPTDWRSDPAPVATMDVGTEWLESESSVGLLVPSTLVPSENNLLVNPHHKDFPACLASVKPLSFVFDPRLK

Download         Length: 459 bp

>T141301 NZ_CP059917:5116954-5117265 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16401.94 Da        Isoelectric Point: 9.7155

>AT141301 WP_002210982.1 NZ_CP045149:c1919729-1919283 [Yersinia pestis]
MRAYRPNPATKMGTLWREIGLPASRGTILVDSIKMGFSVDVIDSIHLWASMPKAEILRATGIPSRSLTRRRTHDGRFTPE
ESERIARFVRVMDAAVDLFGGDKGKAITWMSTPIKGLGYRSPDSLLETETGALEVCDLIGRLEHGVFT

Download         Length: 447 bp

>AT141301 NZ_CP059917:5117266-5117556 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E8XJV6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8XFL1

References