Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 908808..909428 Replicon chromosome
Accession NZ_CP045149
Organism Yersinia pestis strain SCPM-O-DNA-18 (I-3113)

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag GCK70_RS04325 Protein ID WP_002208622.1
Coordinates 908808..909011 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag GCK70_RS04330 Protein ID WP_002218472.1
Coordinates 909060..909428 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK70_RS04295 903830..904168 + 339 WP_002208627.1 P-II family nitrogen regulator -
GCK70_RS04300 904208..905497 + 1290 WP_002228344.1 ammonium transporter AmtB -
GCK70_RS04305 905757..906617 - 861 WP_002208625.1 acyl-CoA thioesterase II -
GCK70_RS04310 906874..907395 + 522 WP_002208624.1 lipoprotein -
GCK70_RS04315 907531..907920 - 390 WP_002208623.1 MGMT family protein -
GCK70_RS04325 908808..909011 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
GCK70_RS04330 909060..909428 - 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
GCK70_RS04335 909587..909940 - 354 WP_002208619.1 hypothetical protein -
GCK70_RS04340 910814..910957 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
GCK70_RS04345 910973..911233 - 261 WP_002208617.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T141300 WP_002208622.1 NZ_CP045149:c909011-908808 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T141300 NZ_CP059917:c4621052-4620945 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT141300 WP_002218472.1 NZ_CP045149:c909428-909060 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT141300 NZ_CP059917:4621101-4621167 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References