Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /COG5642-COG5654
Location 2166687..2167653 Replicon chromosome
Accession NZ_CP045145
Organism Yersinia pestis strain SCPM-O-B-6899 (231)

Toxin (Protein)


Gene name - Uniprot ID A0A0E8XJV6
Locus tag GCK69_RS10105 Protein ID WP_002210981.1
Coordinates 2166687..2167145 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q66A69
Locus tag GCK69_RS10110 Protein ID WP_002210982.1
Coordinates 2167207..2167653 (-) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK69_RS10075 2162501..2163166 + 666 WP_002210975.1 phage shock protein PspA -
GCK69_RS10080 2163328..2163555 + 228 WP_002210976.1 envelope stress response membrane protein PspB -
GCK69_RS10085 2163555..2163914 + 360 WP_002210977.1 envelope stress response membrane protein PspC -
GCK69_RS10090 2163999..2164241 + 243 WP_002216375.1 phage shock protein PspD -
GCK69_RS10095 2164222..2165619 + 1398 WP_002210979.1 YcjX family protein -
GCK69_RS10100 2165616..2166677 + 1062 WP_002210980.1 YcjF family protein -
GCK69_RS10105 2166687..2167145 - 459 WP_002210981.1 RES family NAD+ phosphorylase Toxin
GCK69_RS10110 2167207..2167653 - 447 WP_002210982.1 DUF2384 domain-containing protein Antitoxin
GCK69_RS10115 2167989..2169566 + 1578 WP_002210983.1 transcriptional regulator TyrR -
GCK69_RS10120 2169713..2170171 - 459 WP_002213775.1 IS200/IS605-like element IS1541B family transposase -
GCK69_RS10125 2170424..2170927 - 504 WP_002210984.1 thiol peroxidase -
GCK69_RS10130 2171090..2172064 + 975 WP_002210985.1 L-Ala-D/L-Glu epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2169713..2170171 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(2-147)

Antitoxin

Antitoxin Xre

(15-147)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 16994.38 Da        Isoelectric Point: 4.8732

>T141286 WP_002210981.1 NZ_CP045145:c2167145-2166687 [Yersinia pestis]
MTLYRIVMRRYLASAWTGYGAEIYGGRWNHKGHAAIYLASSISLAMLETLAHIQDSSTLSEFELFQIEISDSSIMLLQPQ
DWPTDWRSDPAPVATMDVGTEWLESESSVGLLVPSTLVPSENNLLVNPHHKDFPACLASVKPLSFVFDPRLK

Download         Length: 459 bp

>T141286 NZ_CP059917:c2190311-2190117 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGTCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTTGAAAGATGGAATAAACGAA
CCACTGGAAATAATAACGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16401.94 Da        Isoelectric Point: 9.7155

>AT141286 WP_002210982.1 NZ_CP045145:c2167653-2167207 [Yersinia pestis]
MRAYRPNPATKMGTLWREIGLPASRGTILVDSIKMGFSVDVIDSIHLWASMPKAEILRATGIPSRSLTRRRTHDGRFTPE
ESERIARFVRVMDAAVDLFGGDKGKAITWMSTPIKGLGYRSPDSLLETETGALEVCDLIGRLEHGVFT

Download         Length: 447 bp

>AT141286 NZ_CP059917:2189940-2190118 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E8XJV6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8XFL1

References