Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1154821..1155441 Replicon chromosome
Accession NZ_CP045145
Organism Yersinia pestis strain SCPM-O-B-6899 (231)

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag GCK69_RS05340 Protein ID WP_002208622.1
Coordinates 1154821..1155024 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag GCK69_RS05345 Protein ID WP_002218472.1
Coordinates 1155073..1155441 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCK69_RS05310 1149843..1150181 + 339 WP_002208627.1 P-II family nitrogen regulator -
GCK69_RS05315 1150221..1151510 + 1290 WP_002228344.1 ammonium transporter AmtB -
GCK69_RS05320 1151770..1152630 - 861 WP_002208625.1 acyl-CoA thioesterase II -
GCK69_RS05325 1152887..1153408 + 522 WP_002208624.1 lipoprotein -
GCK69_RS05330 1153544..1153933 - 390 WP_002208623.1 MGMT family protein -
GCK69_RS05340 1154821..1155024 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
GCK69_RS05345 1155073..1155441 - 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
GCK69_RS05350 1155600..1155953 - 354 WP_002208619.1 hypothetical protein -
GCK69_RS05355 1156827..1156970 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
GCK69_RS05360 1156986..1157246 - 261 WP_002208617.1 type B 50S ribosomal protein L31 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-64)

Antitoxin

(1-118)


Sequences


Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T141285 WP_002208622.1 NZ_CP045145:c1155024-1154821 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T141285 NZ_CP059917:c2043258-2043151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT141285 WP_002218472.1 NZ_CP045145:c1155441-1155073 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT141285 NZ_CP059917:2043311-2043372 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 2K5S


Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References