Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsaAT/-
Location 2114391..2114859 Replicon chromosome
Accession NZ_CP045137
Organism Staphylococcus haemolyticus strain VB5326

Toxin (Protein)


Gene name TsaT Uniprot ID Q4L4Q4
Locus tag GA597_RS10480 Protein ID WP_011276326.1
Coordinates 2114671..2114859 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name TsaA Uniprot ID Q4L4Q5
Locus tag GA597_RS10475 Protein ID WP_011276325.1
Coordinates 2114391..2114648 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GA597_RS10440 (GA597_06350) 2109567..2110292 - 726 WP_033079650.1 type I 3-dehydroquinate dehydratase -
GA597_RS10445 (GA597_06345) 2110455..2110877 + 423 WP_016931015.1 organic hydroperoxide resistance protein -
GA597_RS10450 (GA597_06340) 2111140..2111631 + 492 WP_033079649.1 GNAT family N-acetyltransferase -
GA597_RS10455 (GA597_06335) 2111763..2112380 + 618 WP_033079648.1 LysE/ArgO family amino acid transporter -
GA597_RS10460 (GA597_06330) 2112567..2113175 - 609 WP_011276323.1 histidine phosphatase family protein -
GA597_RS10465 (GA597_06325) 2113403..2113645 + 243 WP_011276324.1 hypothetical protein -
GA597_RS10470 (GA597_06320) 2113637..2113840 - 204 WP_033079647.1 sterile alpha motif-like domain-containing protein -
GA597_RS10475 (GA597_06315) 2114391..2114648 + 258 WP_011276325.1 hypothetical protein Antitoxin
GA597_RS10480 (GA597_06310) 2114671..2114859 + 189 WP_011276326.1 hypothetical protein Toxin
GA597_RS10485 (GA597_06305) 2114977..2115558 + 582 WP_033079646.1 lipoprotein N-acylation protein LnsA -
GA597_RS10490 (GA597_06300) 2115606..2115842 - 237 WP_016931008.1 hypothetical protein -
GA597_RS10495 (GA597_06295) 2116012..2116758 + 747 WP_033079645.1 membrane lipoprotein lipid attachment site-containing protein -
GA597_RS10500 (GA597_06290) 2116827..2117435 - 609 WP_033079644.1 GNAT family N-acetyltransferase -
GA597_RS10505 (GA597_06285) 2117572..2118318 + 747 WP_119890370.1 poly-gamma-glutamate hydrolase family protein -
GA597_RS10510 (GA597_06280) 2118394..2118681 + 288 WP_016931004.1 hypothetical protein -
GA597_RS10515 (GA597_06275) 2118920..2119144 + 225 WP_016931003.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage fusB - 2113403..2180195 66792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7110.80 Da        Isoelectric Point: 9.6269

>T141276 WP_011276326.1 NZ_CP045137:2114671-2114859 [Staphylococcus haemolyticus]
MSLHLTILLWLGIIFVIAASIILGLLLKSKKEERKESYLGFTVIFYIFGFALLIYVFIFGIL

Download         Length: 189 bp

>T141276 NZ_CP059917:284406-284579 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9914.97 Da        Isoelectric Point: 4.0625

>AT141276 WP_011276325.1 NZ_CP045137:2114391-2114648 [Staphylococcus haemolyticus]
MNNNKIFWINIFMTLLFLGFNIIVTYNADLDDFFWLIPGLTISGITIVLSLSTALICKNLVSEVIFLINIVMLLYYIYPM
VYTFF

Download         Length: 258 bp

>AT141276 NZ_CP059917:284082-284378 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A1KAB5

Antitoxin

Source ID Structure
AlphaFold DB A0A2A1KAX9

References