Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 969208..969740 Replicon chromosome
Accession NZ_CP045137
Organism Staphylococcus haemolyticus strain VB5326

Toxin (Protein)


Gene name mazF Uniprot ID Q4L7V3
Locus tag GA597_RS04845 Protein ID WP_011275272.1
Coordinates 969375..969740 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q4L7V4
Locus tag GA597_RS04840 Protein ID WP_011275271.1
Coordinates 969208..969378 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GA597_RS04815 (GA597_11970) 964994..965473 + 480 WP_011275266.1 PH domain-containing protein -
GA597_RS04820 (GA597_11965) 965466..966989 + 1524 WP_016931363.1 PH domain-containing protein -
GA597_RS04825 (GA597_11960) 966982..967509 + 528 WP_033080076.1 PH domain-containing protein -
GA597_RS04830 (GA597_11955) 967519..967878 + 360 WP_011275269.1 holo-ACP synthase -
GA597_RS04835 (GA597_11950) 967974..969122 + 1149 WP_016931365.1 alanine racemase -
GA597_RS04840 (GA597_11945) 969208..969378 + 171 WP_011275271.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GA597_RS04845 (GA597_11940) 969375..969740 + 366 WP_011275272.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GA597_RS04850 (GA597_11935) 970048..971049 + 1002 WP_011275273.1 PP2C family protein-serine/threonine phosphatase -
GA597_RS04855 (GA597_11930) 971148..971474 + 327 WP_011275274.1 anti-sigma factor antagonist -
GA597_RS04860 (GA597_11925) 971503..971955 + 453 WP_046309853.1 anti-sigma B factor RsbW -
GA597_RS04865 (GA597_11920) 971930..972700 + 771 WP_011275276.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13672.75 Da        Isoelectric Point: 8.4121

>T141274 WP_011275272.1 NZ_CP045137:969375-969740 [Staphylococcus haemolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYRLDKDSVILLEQIR
TLDKKRLKEKLTYLSDEKMQEVDEALDISLGLHDEVKTQNT

Download         Length: 366 bp

>T141274 NZ_CP059908:187473-187778 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6380.08 Da        Isoelectric Point: 4.0325

>AT141274 WP_011275271.1 NZ_CP045137:969208-969378 [Staphylococcus haemolyticus]
MLTFNQNRSHSLEQSLKEGYAQMAELNLSLATEAFPLECEACDCNETYLISNYKSE

Download         Length: 171 bp

>AT141274 NZ_CP059908:187253-187471 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A1KA75

Antitoxin

Source ID Structure
AlphaFold DB A0A2A1K7K7

References