Detailed information of TA system TA141250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/NGN_SP-Phd
• Location: 432059..432719
• Replicon: chromosome
• Accession: NZ_CP045119
• Organism: Rubrobacter sp. SCSIO 52909

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GBA63_RS01980
• Protein ID: WP_166172981.1
• Coordinates: 432303..432719 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GBA63_RS01975
• Protein ID: WP_166172979.1
• Coordinates: 432059..432301 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBA63_RS01955	428995..429411	+	417	WP_166172973.1	hypothetical protein	-
GBA63_RS01960	429432..429905	-	474	WP_166172975.1	hypothetical protein	-
GBA63_RS01965	430199..431347	+	1149	WP_166172977.1	DEAD/DEAH box helicase	-
GBA63_RS01970	431368..431895	-	528	WP_207957027.1	cupin domain-containing protein	-
GBA63_RS01975	432059..432301	+	243	WP_166172979.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GBA63_RS01980	432303..432719	+	417	WP_166172981.1	type II toxin-antitoxin system VapC family toxin	Toxin
GBA63_RS01985	432808..433788	+	981	WP_166172983.1	HNH endonuclease	-
GBA63_RS01990	433791..437060	-	3270	WP_166172985.1	DNA polymerase III subunit alpha	-
GBA63_RS01995	437060..437479	-	420	WP_166172987.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-63)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15444.68 Da
• Isoelectric Point: 5.2872

>T141250 WP_166172981.1 NZ_CP045119:432303-432719 [Rubrobacter sp. SCSIO 52909]
VKYLLDTNVIFEARKPRGAERVKRWISSVPVEDLYLSVLTLGEVRRGIGLLEGRDPVQAEVYEAWLTTVLRDYADRIIPV
DAETAEEWGRMNVPGPVPVVDSLMAATARVRNMTFVTRNTSDVARTGVRLLNPFESSG

Nucleotide

• Length: 417 bp

>T141250 NZ_CP059907:1050328-1050705 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9254.55 Da
• Isoelectric Point: 4.8952

>AT141250 WP_166172979.1 NZ_CP045119:432059-432301 [Rubrobacter sp. SCSIO 52909]
MTWQLQEAKQRFSELVRRTLEEGPQVVTRHGKEVVVVVSAEEYGRTGEKKPDFKEFLLSGPDLSVLDLERPKEMPRDVDL

Nucleotide

• Length: 243 bp

>AT141250 NZ_CP059907:1050110-1050331 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA