Detailed information of TA system TA141249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 6241806..6242394
• Replicon: chromosome
• Accession: NZ_CP045118
• Organism: Pseudomonas sp. SCB32

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GA645_RS28430
• Protein ID: WP_152227729.1
• Coordinates: 6241806..6242108 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GA645_RS28435
• Protein ID: WP_152227731.1
• Coordinates: 6242101..6242394 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GA645_RS28410	6237417..6238793	+	1377	WP_152227724.1	L-serine ammonia-lyase	-
GA645_RS28415	6239167..6240273	+	1107	WP_152227726.1	GlxA family transcriptional regulator	-
GA645_RS28890	6240599..6241075	-	477	WP_178119601.1	Bro-N domain-containing protein	-
GA645_RS28425	6241438..6241509	-	72	Protein_5569	VOC family protein	-
GA645_RS28430	6241806..6242108	+	303	WP_152227729.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GA645_RS28435	6242101..6242394	+	294	WP_152227731.1	putative addiction module antidote protein	Antitoxin
GA645_RS28440	6242848..6243327	-	480	WP_152227733.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (7-93)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11096.72 Da
• Isoelectric Point: 10.0983

>T141249 WP_152227729.1 NZ_CP045118:6241806-6242108 [Pseudomonas sp. SCB32]
MKKIESGKFRAWLHKLKDDTGRARIVARINRLMEGLPGDVAPVGPGVSELRIHHGPGYRVYFHQQGDTLVILLCGGDKGS
QSRDIEAAQQVLAAWRAQHD

Nucleotide

• Length: 303 bp

>T141249 NZ_CP059907:1022834-1023109 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10673.22 Da
• Isoelectric Point: 4.4878

>AT141249 WP_152227731.1 NZ_CP045118:6242101-6242394 [Pseudomonas sp. SCB32]
MTETFTEFEPAEYLTNPEAIAEFMTDALETGDAAYIAKAMGVVARAKGMTELARETGLSREQLYRSFSEQGNPTLKTLLA
VMKALGIEMTIRPQVAH

Nucleotide

• Length: 294 bp

>AT141249 NZ_CP059907:1023106-1023465 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA