Detailed information of TA system TA141245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1730896..1732384
• Replicon: chromosome
• Accession: NZ_CP045118
• Organism: Pseudomonas sp. SCB32

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GA645_RS08205
• Protein ID: WP_152221663.1
• Coordinates: 1730896..1731465 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GA645_RS08210
• Protein ID: WP_152221665.1
• Coordinates: 1731533..1732384 (+)
• Length: 284 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GA645_RS08195	1726555..1727013	+	459	WP_152221659.1	DMT family transporter	-
GA645_RS08200	1727157..1730633	-	3477	WP_152221661.1	hybrid sensor histidine kinase/response regulator	-
GA645_RS08205	1730896..1731465	+	570	WP_152221663.1	GNAT family N-acetyltransferase	Toxin
GA645_RS08210	1731533..1732384	+	852	WP_152221665.1	AraC family transcriptional regulator	Antitoxin
GA645_RS08215	1732674..1734548	+	1875	WP_152221666.1	MFS transporter	-
GA645_RS08220	1734612..1734821	-	210	WP_045210321.1	cold-shock protein	-
GA645_RS08225	1735025..1735339	-	315	WP_152221668.1	quaternary ammonium compound efflux SMR transporter SugE	-
GA645_RS08230	1735474..1736430	-	957	WP_152221670.1	bile acid:sodium symporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (20-141)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21206.40 Da
• Isoelectric Point: 9.6199

>T141245 WP_152221663.1 NZ_CP045118:1730896-1731465 [Pseudomonas sp. SCB32]
MFRPLPITLQRGALRLEPMVEADVPELVTLAEANRDVLQFMSAPGRPDWYRQGLADQREGRALPLVVRLGNEIVGTTRFT
DFLPALPACEIGATWLDQAQHGSGLNATMKYLMLRHAFDSWKMVRVQFKTAASNLRSQRAIEKLGAVREGVLRNHRRLAG
GRLDDTILYSITDHEWPQVKAALEASFNG

Nucleotide

• Length: 570 bp

>T141245 NZ_CP059907:941527-941634 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 284 a.a.
• Molecular weight: 31328.57 Da
• Isoelectric Point: 7.6558

>AT141245 WP_152221665.1 NZ_CP045118:1731533-1732384 [Pseudomonas sp. SCB32]
MTDHHASGERTRFWRAQELGGVELLHARYIEQVFSPHVHEGFAFVMIEQGAQRFHHRGAEHFAPAGSVVLINPDEVHTGS
KADEAGWRYRGFYPELAQVSGVLEELELNGGGTPSFDASVVHDPELVRALFAAHRLVDSDAPVLQRQTCWREALLLLFQR
HARIPDAPPAGNEPVAVARARELLGSRLMEPPSLEELAAAVGLSPFHFARVFRKATGLPPHAWLKQRRLEQARALLKQGC
APVGVAMQLGFADQSHLSRQFKQAYGVGPGEYRQACARSFKTA

Nucleotide

• Length: 852 bp

>AT141245 NZ_CP059907:c941478-941412 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC