Detailed information of TA system TA141233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3052463..3053112
• Replicon: chromosome
• Accession: NZ_CP045103
• Organism: Acinetobacter johnsonii strain AJ413

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GBN93_RS14610
• Protein ID: WP_234964794.1
• Coordinates: 3052463..3052882 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0U3RE63
• Locus tag: GBN93_RS14615
• Protein ID: WP_010327422.1
• Coordinates: 3052882..3053112 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN93_RS14575 (GBN93_14640)	3048335..3048730	-	396	WP_010327416.1	VOC family protein	-
GBN93_RS14580 (GBN93_14645)	3049320..3049592	-	273	WP_234964789.1	hypothetical protein	-
GBN93_RS14585 (GBN93_14650)	3049701..3050408	-	708	WP_234964790.1	hypothetical protein	-
GBN93_RS14590 (GBN93_14655)	3050621..3050974	+	354	WP_234964791.1	hypothetical protein	-
GBN93_RS14595 (GBN93_14660)	3051056..3051262	-	207	WP_234964792.1	hypothetical protein	-
GBN93_RS14600 (GBN93_14665)	3051495..3052070	-	576	WP_234964793.1	DUF4126 domain-containing protein	-
GBN93_RS14605 (GBN93_14670)	3052189..3052332	-	144	WP_004695201.1	zinc ribbon-containing protein	-
GBN93_RS14610 (GBN93_14675)	3052463..3052882	-	420	WP_234964794.1	type II toxin-antitoxin system VapC family toxin	Toxin
GBN93_RS14615 (GBN93_14680)	3052882..3053112	-	231	WP_010327422.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GBN93_RS14620 (GBN93_14685)	3053185..3054123	-	939	WP_010327423.1	hypothetical protein	-
GBN93_RS14625 (GBN93_14690)	3054128..3054349	-	222	WP_010327424.1	hypothetical protein	-
GBN93_RS14630 (GBN93_14695)	3054429..3057035	-	2607	WP_234964795.1	Fe/S-dependent 2-methylisocitrate dehydratase AcnD	-
GBN93_RS14635 (GBN93_14700)	3057297..3058019	+	723	WP_234964796.1	folate-binding Fe/S cluster repair protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-128)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16153.51 Da
• Isoelectric Point: 6.7559

>T141233 WP_234964794.1 NZ_CP045103:c3052882-3052463 [Acinetobacter johnsonii]
MQTQYLLDTNICIYIIKHQPEIVRQHFEKHLPHRNILISVITLGELRFGAEKSQQKEKALKVIDKLTSMIQVVELDEYVA
DHYAQIRQDLSSKGQIIGANDLWLAAHARSNNWVMVTNNEKEFLRVDGLQIENWTSNTL

Nucleotide

• Length: 420 bp

>T141233 NZ_CP059900:3007393-3007674 [Tomitella sp. HY172]
GTGATCCGGTCCTTCAAGAACACCGACACAGAGAAGGTCTGGAACCGTGAACACGTCCGAGCCCTCGGACCCGAACTGCA
ACGCGCCGCGCAGAAGACGCTCCGCCTCCTCAACGCGGCCTCAGAATTGAACAGCCTGCGAGTGCCGCCCGGCAACCGCC
TGGAGAAGCTGGTCGGCGACCGAGCCGGACAGCACTCCATCCGCATCAACGACCAATACCGGATCTGCTTCGTTTGGACC
GACAACGGCGCAGACGACGTCGAGATCACCGACTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9023.27 Da
• Isoelectric Point: 4.6936

>AT141233 WP_010327422.1 NZ_CP045103:c3053112-3052882 [Acinetobacter johnsonii]
MTTAKVFMTGRSQAVRLPKEFRFEGTEVEIFRRGDDVILREKPVTVDRLFKVLAQMPNDFYAEERVDEPPQEREEF

Nucleotide

• Length: 231 bp

>AT141233 NZ_CP059900:3007694-3007999 [Tomitella sp. HY172]
ATGGCTGAGACGACCAAGGCACACGACCCGATCACTCCCGGCGAGATCCTGCTGACCGAGTTCTTGGAGCCGCTGGGCAT
CACCCAGTACCGCCTCGCCCAGGCCACCGGGCTTCCCCAGACCCGGATCAGCGAGATCGTGCGCGGCAAGCGAGCCATCA
CCATCGACACTGCACTGCGACTCTCCAAGACCCTCGGCGTCGACGACCGCTTCTGGATCAACATCCAGACCGACTACGAC
CTCGAGGTCGAGCGCGACCTGCACGCCGACGAACTCGCCAAGGTCACCCCCCTGGTCGCGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0U3RE63