Detailed information of TA system TA141225

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2092773..2093433
• Replicon: chromosome
• Accession: NZ_CP045098
• Organism: Pectobacterium carotovorum strain ZM1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: GBN63_RS09525
• Protein ID: WP_010301815.1
• Coordinates: 2093059..2093433 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1V2R9A9
• Locus tag: GBN63_RS09520
• Protein ID: WP_039355397.1
• Coordinates: 2092773..2093039 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN63_RS09500 (GBN63_09515)	2088888..2089892	-	1005	WP_039470792.1	LacI family DNA-binding transcriptional regulator	-
GBN63_RS09505 (GBN63_09520)	2089947..2090555	-	609	WP_010301802.1	HD domain-containing protein	-
GBN63_RS09510 (GBN63_09525)	2090834..2091481	+	648	WP_039539856.1	hemolysin III family protein	-
GBN63_RS09515 (GBN63_09530)	2091536..2092537	-	1002	WP_161508817.1	tRNA-modifying protein YgfZ	-
GBN63_RS09520 (GBN63_09535)	2092773..2093039	+	267	WP_039355397.1	FAD assembly factor SdhE	Antitoxin
GBN63_RS09525 (GBN63_09540)	2093059..2093433	+	375	WP_010301815.1	protein YgfX	Toxin
GBN63_RS09530 (GBN63_09545)	2093502..2094437	+	936	WP_010301818.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
GBN63_RS09535 (GBN63_09550)	2094503..2094826	-	324	WP_010301820.1	hypothetical protein	-
GBN63_RS09540 (GBN63_09555)	2094855..2095373	-	519	WP_039470802.1	flavodoxin FldB	-
GBN63_RS09545 (GBN63_09560)	2095713..2096099	-	387	WP_010301824.1	thioesterase family protein	-
GBN63_RS09550 (GBN63_09565)	2096307..2097635	-	1329	WP_010301826.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14654.33 Da
• Isoelectric Point: 10.5560

>T141225 WP_010301815.1 NZ_CP045098:2093059-2093433 [Pectobacterium carotovorum]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 375 bp

>T141225 NZ_CP059898:2978645-2978896 [Streptomyces sp. M54]
ATGCGCACGATGACCTACACCGAGTCCCGCGCGAAGTACGCCGAGACTCTGAGCTCGGTCGTCGACGATCGCGAGGAGGT
CGTCGTCACGCGCGCCGGACACGAACCCGTGGTGATAGTGGCGCTCGACGAATACGAGTCCCTGAAGGAGACCGCGTACC
AGCTCAGGAGCCCCGAGAACGCACGCCGCCTGCTGGCCTCGATCGACCGCCTGGAGAACGGCGGCGGCACCGTGCGGGAG
CTCGCGGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10506.99 Da
• Isoelectric Point: 6.2223

>AT141225 WP_039355397.1 NZ_CP045098:2092773-2093039 [Pectobacterium carotovorum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT141225 NZ_CP059898:2978893-2979153 [Streptomyces sp. M54]
GTGAAGTTCGTATGGGACCAGTCGTCCTGGGACGACTACGTCTGGTGGCAGAACCAGGACCGCAAGATCCTCAAGCGGAT
CAACGCGCTGCTCCAGGACATCGCCCGGAACGGCAACGAGGGGATGGGCAAGCCGGACCCCTTGAAGCACGGCTTCCACG
GATACTGGTCGCGGCGCATCACCGGCGAACACCGGCTCATCTACAAGGTGGGGGACGACGAGGTGCGCGTCGCGGCCTGC
CGTTACCACTACGGACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2R9A9