Detailed information of TA system TA141222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 1737382..1738221
• Replicon: chromosome
• Accession: NZ_CP045098
• Organism: Pectobacterium carotovorum strain ZM1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7V8PT96
• Locus tag: GBN63_RS07830
• Protein ID: WP_039535732.1
• Coordinates: 1737859..1738221 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBN63_RS07825
• Protein ID: WP_039470394.1
• Coordinates: 1737382..1737855 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN63_RS07810 (GBN63_07825)	1732696..1733619	-	924	WP_239768027.1	aminoimidazole riboside kinase	-
GBN63_RS07815 (GBN63_07830)	1734092..1734643	+	552	WP_201962112.1	polymer-forming cytoskeletal protein	-
GBN63_RS07820 (GBN63_07835)	1734675..1737392	+	2718	WP_239768029.1	CRISPR-associated helicase Cas3'	-
GBN63_RS07825 (GBN63_07840)	1737382..1737855	-	474	WP_039470394.1	helix-turn-helix domain-containing protein	Antitoxin
GBN63_RS07830 (GBN63_07845)	1737859..1738221	-	363	WP_039535732.1	type II toxin-antitoxin system HigB family toxin	Toxin
GBN63_RS07835 (GBN63_07850)	1738835..1740403	+	1569	WP_239768031.1	type I-E CRISPR-associated protein Cse1/CasA	-
GBN63_RS07840 (GBN63_07855)	1740387..1741034	+	648	WP_161508712.1	type I-E CRISPR-associated protein Cse2/CasB	-
GBN63_RS07845 (GBN63_07860)	1741038..1742105	+	1068	WP_203470503.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
GBN63_RS07850 (GBN63_07865)	1742115..1742849	+	735	WP_010308679.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1737382..1748043	10661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Antitoxin HigA (43-157)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14161.38 Da
• Isoelectric Point: 9.7305

>T141222 WP_039535732.1 NZ_CP045098:c1738221-1737859 [Pectobacterium carotovorum]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFALLENESRKGPLDIYVFDIN
GNNCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T141222 NZ_CP059896:c2403751-2403464 [Ciceribacter thiooxidans]
ATGATCGAGATCCGGCAGCATCCCGATTTCGCCGGCTGGCTTCGCAAGCTAAAGGACCGGCAGGCGCAGACACGCATCGC
GATCCGTCTGGTGCGGCTTCAGTCCGGCCTGATGGGCGACGTGAAGTATTTCGGCGGCATCGGCGAAGTGCGGATCGATT
ACGGCCCCGGCTATCGGCTCTATTTCGTGAGGCGCGGCGAGGCGCTCATCATCCTGCTGTGCGGCGGCGACAAGGCGAGC
CAGGAAAAAGACATCCGCCGCGCAATGGATCTGGCAAAGGACGTGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17763.02 Da
• Isoelectric Point: 4.2043

>AT141222 WP_039470394.1 NZ_CP045098:c1737855-1737382 [Pectobacterium carotovorum]
MRNTSLGSNSSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLYLYSSDVNDIPATFAELI
ADRVYEYEKKHRDVPHVSQAEALAFFIEERGLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT141222 NZ_CP059896:c2403462-2403163 [Ciceribacter thiooxidans]
ATGGCGACGGAAACCATCCCCTTCGATGCTTCGGAATTCTTTGCCGATGCCGAAGCCCAGGCGGCGCTGCTGACGGATGC
GTTCGAAAGCGGCGATGCGGCCTACATCGCCAATGCCCTCGGGATCATCGCCAAAGCCAAGGGCATGACGCGCGTCGCCC
GGGAGGCGGGGGTGACTCGTGAGGCCCTCTACAAGGCGCTGAGCGAGCGCGGCGATCCCAAATTCTCCACGATTCTCGGC
GTTGCGAAGGCGCTCGGCCTGAAATTCACCGTGACGCCGGCCTCCGGGCCGGCGGAATAG

Structures

Toxin

• AlphaFold DB: A0A7V8PT96