Detailed information of TA system TA141221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1451840..1452447
• Replicon: chromosome
• Accession: NZ_CP045098
• Organism: Pectobacterium carotovorum strain ZM1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GBN63_RS06515
• Protein ID: WP_239767934.1
• Coordinates: 1451840..1452229 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: C6DIE5
• Locus tag: GBN63_RS06520
• Protein ID: WP_010300679.1
• Coordinates: 1452226..1452447 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN63_RS06485 (GBN63_06495)	1447147..1448361	+	1215	WP_239767927.1	AI-2E family transporter	-
GBN63_RS06490 (GBN63_06500)	1448372..1448734	-	363	WP_239767929.1	DUF596 domain-containing protein	-
GBN63_RS06495 (GBN63_06505)	1448777..1449163	-	387	WP_174447492.1	hypothetical protein	-
GBN63_RS06500 (GBN63_06510)	1449277..1449822	+	546	WP_201878363.1	hypothetical protein	-
GBN63_RS06505 (GBN63_06515)	1449870..1450427	-	558	WP_239767931.1	hypothetical protein	-
GBN63_RS06510 (GBN63_06520)	1450706..1451500	-	795	WP_239767933.1	class I SAM-dependent methyltransferase	-
GBN63_RS06515 (GBN63_06525)	1451840..1452229	-	390	WP_239767934.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GBN63_RS06520 (GBN63_06530)	1452226..1452447	-	222	WP_010300679.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GBN63_RS06525 (GBN63_06535)	1452496..1452819	-	324	WP_239767936.1	hypothetical protein	-
GBN63_RS06530 (GBN63_06540)	1453164..1453526	+	363	WP_161502993.1	hypothetical protein	-
GBN63_RS06535 (GBN63_06545)	1454086..1455018	-	933	WP_239767938.1	hypothetical protein	-
GBN63_RS06540 (GBN63_06550)	1455132..1455365	-	234	WP_239767940.1	AlpA family transcriptional regulator	-
GBN63_RS06545 (GBN63_06555)	1455610..1456185	+	576	WP_239767941.1	hypothetical protein	-
GBN63_RS06550 (GBN63_06560)	1456221..1456598	-	378	WP_239767943.1	helix-turn-helix transcriptional regulator	-
GBN63_RS06555 (GBN63_06565)	1456838..1457437	+	600	WP_239767945.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1451840..1469226	17386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14611.48 Da
• Isoelectric Point: 6.3291

>T141221 WP_239767934.1 NZ_CP045098:c1452229-1451840 [Pectobacterium carotovorum]
MNWVSAQEVIAFHDRILRRLPGVAGMPDPGRAEAIIYRVQNRTHYEGITDVFELAATYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLVRDHDNTLENLTVEAATGAKNVDQLAQHLRNLEDEISPT

Nucleotide

• Length: 390 bp

>T141221 NZ_CP059896:c2403462-2403163 [Ciceribacter thiooxidans]
ATGGCGACGGAAACCATCCCCTTCGATGCTTCGGAATTCTTTGCCGATGCCGAAGCCCAGGCGGCGCTGCTGACGGATGC
GTTCGAAAGCGGCGATGCGGCCTACATCGCCAATGCCCTCGGGATCATCGCCAAAGCCAAGGGCATGACGCGCGTCGCCC
GGGAGGCGGGGGTGACTCGTGAGGCCCTCTACAAGGCGCTGAGCGAGCGCGGCGATCCCAAATTCTCCACGATTCTCGGC
GTTGCGAAGGCGCTCGGCCTGAAATTCACCGTGACGCCGGCCTCCGGGCCGGCGGAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.01 Da
• Isoelectric Point: 5.0359

>AT141221 WP_010300679.1 NZ_CP045098:c1452447-1452226 [Pectobacterium carotovorum]
MRTYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT141221 NZ_CP059896:c2403751-2403464 [Ciceribacter thiooxidans]
ATGATCGAGATCCGGCAGCATCCCGATTTCGCCGGCTGGCTTCGCAAGCTAAAGGACCGGCAGGCGCAGACACGCATCGC
GATCCGTCTGGTGCGGCTTCAGTCCGGCCTGATGGGCGACGTGAAGTATTTCGGCGGCATCGGCGAAGTGCGGATCGATT
ACGGCCCCGGCTATCGGCTCTATTTCGTGAGGCGCGGCGAGGCGCTCATCATCCTGCTGTGCGGCGGCGACAAGGCGAGC
CAGGAAAAAGACATCCGCCGCGCAATGGATCTGGCAAAGGACGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A221TFG2