Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1289749..1290366 Replicon chromosome
Accession NZ_CP045098
Organism Pectobacterium carotovorum strain ZM1

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag GBN63_RS05825 Protein ID WP_010281507.1
Coordinates 1290187..1290366 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7T5QP09
Locus tag GBN63_RS05820 Protein ID WP_010299285.1
Coordinates 1289749..1290159 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBN63_RS05805 (GBN63_05815) 1285614..1286657 - 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -
GBN63_RS05810 (GBN63_05820) 1286967..1289165 - 2199 WP_239767860.1 primosomal protein N' -
GBN63_RS05815 (GBN63_05825) 1289437..1289652 + 216 WP_010281510.1 50S ribosomal protein L31 -
GBN63_RS05820 (GBN63_05830) 1289749..1290159 - 411 WP_010299285.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GBN63_RS05825 (GBN63_05835) 1290187..1290366 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
GBN63_RS05830 (GBN63_05840) 1290466..1291476 - 1011 WP_239767862.1 iron ABC transporter permease -
GBN63_RS05835 (GBN63_05845) 1291473..1292435 - 963 WP_239768438.1 ABC transporter substrate-binding protein -
GBN63_RS05840 (GBN63_05850) 1292435..1293241 - 807 WP_239767864.1 ABC transporter ATP-binding protein -
GBN63_RS05845 (GBN63_05855) 1293459..1293776 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
GBN63_RS05850 (GBN63_05860) 1293964..1295124 + 1161 WP_010299271.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T141219 WP_010281507.1 NZ_CP045098:c1290366-1290187 [Pectobacterium carotovorum]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T141219 NZ_CP059891:c139505-139014 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14549.41 Da        Isoelectric Point: 4.3391

>AT141219 WP_010299285.1 NZ_CP045098:c1290159-1289749 [Pectobacterium carotovorum]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKNSEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT141219 NZ_CP059891:c139759-139493 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A7T5QP09

References