Detailed information of TA system TA141216

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3980922..3981647
• Replicon: chromosome
• Accession: NZ_CP045097
• Organism: Pectobacterium carotovorum strain RC5297

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: F9W95_RS17670
• Protein ID: WP_211005891.1
• Coordinates: 3980922..3981242 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: F9W95_RS17675
• Protein ID: WP_211005890.1
• Coordinates: 3981312..3981647 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9W95_RS17640 (F9W95_17715)	3976766..3977644	-	879	WP_240004383.1	cell envelope biogenesis protein OmpA	-
F9W95_RS17645 (F9W95_17725)	3978209..3978643	-	435	Protein_3453	glycosyl hydrolase 108 family protein	-
F9W95_RS17650 (F9W95_17730)	3978643..3978750	-	108	Protein_3454	phage holin family protein	-
F9W95_RS17655 (F9W95_17735)	3978742..3979213	+	472	Protein_3455	integrase	-
F9W95_RS17660 (F9W95_17740)	3979644..3979841	-	198	WP_102118417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
F9W95_RS17665 (F9W95_17745)	3979971..3980804	-	834	WP_233986353.1	DUF4942 domain-containing protein	-
F9W95_RS17670 (F9W95_17750)	3980922..3981242	-	321	WP_211005891.1	TA system toxin CbtA family protein	Toxin
F9W95_RS17675 (F9W95_17755)	3981312..3981647	-	336	WP_211005890.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F9W95_RS17680 (F9W95_17760)	3981687..3982160	-	474	WP_233978453.1	DNA repair protein RadC	-
F9W95_RS17685 (F9W95_17765)	3982288..3982638	-	351	WP_137742056.1	hypothetical protein	-
F9W95_RS17690 (F9W95_17770)	3982695..3983528	-	834	WP_233978451.1	hypothetical protein	-
F9W95_RS17695	3983675..3983833	-	159	WP_154715508.1	hypothetical protein	-
F9W95_RS17700 (F9W95_17775)	3984017..3984904	-	888	WP_233978450.1	50S ribosome-binding GTPase	-
F9W95_RS17705 (F9W95_17780)	3985000..3985734	-	735	WP_240004384.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12081.76 Da
• Isoelectric Point: 4.7768

>T141216 WP_211005891.1 NZ_CP045097:c3981242-3980922 [Pectobacterium carotovorum]
MQTSPAIPPREDNPCASPIAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGVTLVNAVNFLVERYELVRIDRNGF
NWQEQSPFLTAVDVLRARRTTGLLKA

Nucleotide

• Length: 321 bp

>T141216 NZ_CP059890:75061-75477 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12456.25 Da
• Isoelectric Point: 6.8353

>AT141216 WP_211005890.1 NZ_CP045097:c3981647-3981312 [Pectobacterium carotovorum]
MPSSDTPEWGLKCTITPRFGARLVQEGHRLHYLADRASVVGTFSKTEARSLERCFPELIKLLEQKLRTGELNPHQKGCII
LHCNEWTCEADTLGSFGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT141216 NZ_CP059890:74834-75064 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA