Detailed information of TA system TA141215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2723424..2723977
• Replicon: chromosome
• Accession: NZ_CP045097
• Organism: Pectobacterium carotovorum strain RC5297

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7V8IH72
• Locus tag: F9W95_RS11995
• Protein ID: WP_039353430.1
• Coordinates: 2723663..2723977 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: F9W95_RS11990
• Protein ID: WP_039536802.1
• Coordinates: 2723424..2723660 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9W95_RS11960 (F9W95_12030)	2718503..2718748	+	246	WP_119158547.1	YmjA family protein	-
F9W95_RS11965 (F9W95_12035)	2718987..2720018	-	1032	WP_116362863.1	methionine synthase	-
F9W95_RS11970 (F9W95_12040)	2720049..2721026	-	978	WP_240004272.1	DUF1852 domain-containing protein	-
F9W95_RS11975 (F9W95_12045)	2721524..2721868	-	345	WP_116362861.1	gamma-glutamylcyclotransferase family protein	-
F9W95_RS11980 (F9W95_12055)	2722417..2722836	+	420	Protein_2332	2-dehydropantoate 2-reductase N-terminal domain-containing protein	-
F9W95_RS11985 (F9W95_12060)	2722892..2723281	+	390	Protein_2333	ArdC family protein	-
F9W95_RS11990 (F9W95_12065)	2723424..2723660	+	237	WP_039536802.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F9W95_RS11995 (F9W95_12070)	2723663..2723977	+	315	WP_039353430.1	CcdB family protein	Toxin
F9W95_RS12000 (F9W95_12075)	2724011..2724178	-	168	Protein_2336	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
F9W95_RS12005 (F9W95_12085)	2724701..2726194	+	1494	WP_229763820.1	RES family NAD+ phosphorylase	-
F9W95_RS12010 (F9W95_12090)	2726401..2727453	+	1053	WP_116585877.1	MBL fold metallo-hydrolase	-
F9W95_RS12015 (F9W95_12100)	2727660..2728685	+	1026	WP_120358785.1	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2705796..2736599	30803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11549.50 Da
• Isoelectric Point: 7.9859

>T141215 WP_039353430.1 NZ_CP045097:2723663-2723977 [Pectobacterium carotovorum]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T141215 NZ_CP059890:c74026-73721 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8952.03 Da
• Isoelectric Point: 7.1672

>AT141215 WP_039536802.1 NZ_CP045097:2723424-2723660 [Pectobacterium carotovorum]
MTAKPRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT141215 NZ_CP059890:c74246-74028 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7V8IH72