Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2218297..2218922 Replicon chromosome
Accession NZ_CP045097
Organism Pectobacterium carotovorum strain RC5297

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag F9W95_RS09815 Protein ID WP_005976087.1
Coordinates 2218297..2218500 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C6DB73
Locus tag F9W95_RS09820 Protein ID WP_005976089.1
Coordinates 2218554..2218922 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9W95_RS09785 (F9W95_09845) 2213757..2214095 + 339 WP_002208627.1 P-II family nitrogen regulator -
F9W95_RS09790 (F9W95_09850) 2214135..2215421 + 1287 WP_161973278.1 ammonium transporter AmtB -
F9W95_RS09795 (F9W95_09855) 2215556..2216419 - 864 WP_040031226.1 acyl-CoA thioesterase II -
F9W95_RS09800 (F9W95_09860) 2216632..2217213 + 582 WP_103941783.1 YbaY family lipoprotein -
F9W95_RS09805 (F9W95_09865) 2217233..2217553 - 321 WP_015839360.1 MGMT family protein -
F9W95_RS09815 (F9W95_09875) 2218297..2218500 - 204 WP_005976087.1 HHA domain-containing protein Toxin
F9W95_RS09820 (F9W95_09880) 2218554..2218922 - 369 WP_005976089.1 Hha toxicity modulator TomB Antitoxin
F9W95_RS09825 (F9W95_09885) 2219520..2219663 - 144 WP_005976091.1 type B 50S ribosomal protein L36 -
F9W95_RS09830 (F9W95_09890) 2219680..2219928 - 249 WP_039506474.1 type B 50S ribosomal protein L31 -
F9W95_RS09835 (F9W95_09895) 2220164..2223292 - 3129 WP_103862997.1 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T141213 WP_005976087.1 NZ_CP045097:c2218500-2218297 [Pectobacterium carotovorum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T141213 NZ_CP059889:c4989415-4988498 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14032.77 Da        Isoelectric Point: 4.4787

>AT141213 WP_005976089.1 NZ_CP045097:c2218922-2218554 [Pectobacterium carotovorum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Download         Length: 369 bp

>AT141213 NZ_CP059889:c4990179-4989421 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7Z3G5

References