Detailed information of TA system TA141212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1957082..1957608
• Replicon: chromosome
• Accession: NZ_CP045097
• Organism: Pectobacterium carotovorum strain RC5297

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: F9W95_RS08720
• Protein ID: WP_233972553.1
• Coordinates: 1957082..1957387 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: F9W95_RS08725
• Protein ID: WP_015855076.1
• Coordinates: 1957390..1957608 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9W95_RS08695 (F9W95_08745)	1952551..1952844	+	294	WP_019844370.1	DUF1778 domain-containing protein	-
F9W95_RS08700 (F9W95_08750)	1952841..1953329	+	489	WP_233972555.1	GNAT family N-acetyltransferase	-
F9W95_RS08705 (F9W95_08760)	1954190..1955215	-	1026	WP_120358785.1	IS630 family transposase	-
F9W95_RS08710 (F9W95_08765)	1955266..1955505	-	240	WP_240004672.1	RloB family protein	-
F9W95_RS08715 (F9W95_08770)	1955505..1956833	-	1329	WP_233972554.1	ATP-binding protein	-
F9W95_RS08720 (F9W95_08775)	1957082..1957387	-	306	WP_233972553.1	type II toxin-antitoxin system toxin CcdB	Toxin
F9W95_RS08725 (F9W95_08780)	1957390..1957608	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
F9W95_RS08730 (F9W95_08785)	1957667..1958410	-	744	WP_233972552.1	MobC family replication-relaxation protein	-
F9W95_RS08735	1958552..1958722	+	171	WP_240004673.1	hypothetical protein	-
F9W95_RS08740 (F9W95_08795)	1959313..1959654	-	342	WP_180746327.1	hypothetical protein	-
F9W95_RS08745	1959795..1959965	+	171	WP_162847981.1	hypothetical protein	-
F9W95_RS08750 (F9W95_08800)	1960107..1961366	-	1260	WP_233972551.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1936734..1961446	24712
	-	flank	IS/Tn	-	-	1954190..1955215	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11807.63 Da
• Isoelectric Point: 5.1200

>T141212 WP_233972553.1 NZ_CP045097:c1957387-1957082 [Pectobacterium carotovorum]
MQFIVYQYKRTSHYKMFVDVQSDIVETPKRRMVIPLIESHHLSEKVNKTLFPQIRIDGENYRLMTTELSSVPVEVIGETI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T141212 NZ_CP059889:c4833086-4832802 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT141212 WP_015855076.1 NZ_CP045097:c1957608-1957390 [Pectobacterium carotovorum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT141212 NZ_CP059889:c4833318-4833076 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46