Detailed information of TA system TA141204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1089291..1089925
• Replicon: chromosome
• Accession: NZ_CP045097
• Organism: Pectobacterium carotovorum strain RC5297

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: F9W95_RS04835
• Protein ID: WP_103862315.1
• Coordinates: 1089291..1089566 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A221T3Z5
• Locus tag: F9W95_RS04840
• Protein ID: WP_010308714.1
• Coordinates: 1089563..1089925 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9W95_RS04820 (F9W95_04845)	1086318..1087688	-	1371	WP_103862317.1	envelope stress sensor histidine kinase CpxA	-
F9W95_RS04825 (F9W95_04850)	1087685..1088383	-	699	WP_010308725.1	envelope stress response regulator transcription factor CpxR	-
F9W95_RS04830 (F9W95_04855)	1088537..1089061	+	525	WP_103943770.1	cell-envelope stress modulator CpxP	-
F9W95_RS04835 (F9W95_04860)	1089291..1089566	+	276	WP_103862315.1	type II toxin-antitoxin system HicA family toxin	Toxin
F9W95_RS04840 (F9W95_04865)	1089563..1089925	+	363	WP_010308714.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F9W95_RS04845 (F9W95_04870)	1089969..1091162	-	1194	WP_103863058.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1089969..1091162	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10006.60 Da
• Isoelectric Point: 11.1875

>T141204 WP_103862315.1 NZ_CP045097:1089291-1089566 [Pectobacterium carotovorum]
MQGKIASLRKKQRETLSHIFKTPVLSGVKWSSIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T141204 NZ_CP059889:346298-346666 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13495.29 Da
• Isoelectric Point: 4.6225

>AT141204 WP_010308714.1 NZ_CP045097:1089563-1089925 [Pectobacterium carotovorum]
MTKALNTPNTMVVSGQPAIISYVPEIGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERHISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT141204 NZ_CP059889:346080-346301 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A221T3Z5