Detailed information of TA system TA141195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 172149..172786
• Replicon: plasmid pWSGX1
• Accession: NZ_CP045089
• Organism: Burkholderia glumae strain GX

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: GAS19_RS29260
• Protein ID: WP_012735347.1
• Coordinates: 172385..172786 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: GAS19_RS29255
• Protein ID: WP_017924531.1
• Coordinates: 172149..172385 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAS19_RS29240	167449..169284	+	1836	WP_153479182.1	site-specific integrase	-
GAS19_RS29245	169369..169794	+	426	WP_039204392.1	hypothetical protein	-
GAS19_RS29250	170008..171717	+	1710	WP_153479184.1	MCP four helix bundle domain-containing protein	-
GAS19_RS29255	172149..172385	+	237	WP_017924531.1	AbrB family transcriptional regulator	Antitoxin
GAS19_RS29260	172385..172786	+	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
GAS19_RS30745	173035..173364	+	330	WP_155297075.1	hypothetical protein	-
GAS19_RS29270	173382..173612	+	231	WP_153479186.1	hypothetical protein	-
GAS19_RS29275	173645..174373	-	729	WP_012735342.1	IS6 family transposase	-
GAS19_RS29280	174416..175060	+	645	Protein_162	site-specific integrase	-
GAS19_RS29285	175145..175570	+	426	WP_012734109.1	hypothetical protein	-
GAS19_RS29290	175833..176084	+	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GAS19_RS29295	176078..176428	+	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	-
GAS19_RS29300	176487..176681	+	195	WP_124837685.1	hypothetical protein	-
GAS19_RS30750	176831..177052	+	222	WP_017433768.1	hypothetical protein	-
GAS19_RS29305	177275..177739	+	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..201571	201571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T141195 WP_012735347.1 NZ_CP045089:172385-172786 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T141195 NZ_CP059851:c3106203-3105913 [Sandaracinobacter sp. M6]
ATGTTTGAGGTCAGGCGGACGGGCGCATTCATCGACTGGCTGACTGGGTTGAAGGATGTGCAGGGGCGGGCGCGGATTGC
AAAACGCCTTGACCGGCTGGTGGACGGCAATTTCGGGGATGCCAAGTCCGTTGGCGGCGGCGTGAGCGAACTGCGGTTTA
CGTTCGGCCCGGGGTATCGGGTTTATTTCACGGTGCGCGGTCAGGTTGTTGTCATCCTGCTGTGTGGTGGCGACAAGCAG
AGCCAGGTGCGTGACATCGACCGGGCGATCTTGATGGCGAAGGAGGTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8965.10 Da
• Isoelectric Point: 6.3676

>AT141195 WP_017924531.1 NZ_CP045089:172149-172385 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT141195 NZ_CP059851:c3105910-3105629 [Sandaracinobacter sp. M6]
ATGGCGATCGAAACCAAGCCGTGGGATGCCGCCGAGGTGCTGAACACGCCGGCGGACATTGCCGCCTATCTGGATGCCTA
TCTGGAAGATGGCACGCCCGAGGAGCTGCTGCGGGCGGTGAAGGCGATTGCCCGGTCGCGTGGCATGTCCGAGCTGGCGC
GGCAGAGCGGCATCAGCCGGGAGGCGCTGTATCGCGCGTTTGGCGACAAGGGGAATCCCACGTTGGATACGCTGCTGCGG
GTTATGAAGGCGCTGGGGGTTAGGCTGGCCATTGCGGCGTGA

Structures

Toxin

• AlphaFold DB: C5AP38