Detailed information of TA system TA141193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 125543..126315
• Replicon: plasmid pWSGX1
• Accession: NZ_CP045089
• Organism: Burkholderia glumae strain GX

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: GAS19_RS29030
• Protein ID: WP_080942736.1
• Coordinates: 125543..126037 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2J9HHX2
• Locus tag: GAS19_RS29035
• Protein ID: WP_045678699.1
• Coordinates: 126034..126315 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAS19_RS29005	120657..121043	-	387	WP_017432681.1	hypothetical protein	-
GAS19_RS29010	121043..121519	-	477	WP_153479169.1	hypothetical protein	-
GAS19_RS29015	121549..121959	+	411	Protein_109	type VI secretion protein	-
GAS19_RS29020	121969..124890	+	2922	WP_153479171.1	conjugative relaxase	-
GAS19_RS29025	124980..125450	-	471	WP_080942737.1	ProQ activator of osmoprotectant transporter prop	-
GAS19_RS29030	125543..126037	-	495	WP_080942736.1	GNAT family N-acetyltransferase	Toxin
GAS19_RS29035	126034..126315	-	282	WP_045678699.1	DUF1778 domain-containing protein	Antitoxin
GAS19_RS29040	126503..126960	+	458	Protein_114	transposase	-
GAS19_RS29045	127211..127492	+	282	WP_042967384.1	virulence factor	-
GAS19_RS29050	127649..128728	-	1080	WP_017432519.1	MBL fold metallo-hydrolase	-
GAS19_RS29055	128775..129494	-	720	WP_045678698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..201571	201571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17493.08 Da
• Isoelectric Point: 8.6141

>T141193 WP_080942736.1 NZ_CP045089:c126037-125543 [Burkholderia glumae]
MTGWLAPEPLGDAHGLDDFDSGVASLDTWLKRRAFANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGNGVGRALVRDVGLRVLHAAAAIGIRGLIVHALTDSAKAFYERVGFEASPIDPMLLLITLADL
EHAL

Nucleotide

• Length: 495 bp

>T141193 NZ_CP059851:3099239-3099547 [Sandaracinobacter sp. M6]
GTGACCATGACCCAGCTTGCGACCACCCCATTCGACGCCGCCAAATATATCACCGAGCCCGCGGATGTGGTCGACTTCCT
CAACGACGCGTTCGACAGCGGCCATGCCCCCTACATCGCCAAAGTGCTGGGCATCGTCGCGCGCAGCGAAGGCATGTCGA
AAATCGCCGACAAGACCGGCGTCAACCGTCAGGCCCTCTACACCGCGCTCAGCGAGCAGGGCAATCCGACGCTGGAAACG
CTCCTCAAGGTCCTGTCCGCCCTCGGCATGAAACTGAAATGCGAACCCGACCTGGCGAAAGCAGCATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10112.39 Da
• Isoelectric Point: 7.0104

>AT141193 WP_045678699.1 NZ_CP045089:c126315-126034 [Burkholderia glumae]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDAPAQPNAALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT141193 NZ_CP059851:3098901-3099242 [Sandaracinobacter sp. M6]
ATGTATATCATTGAGCGCACCGTCGTCTTTGATGGGTGGCTCGATGGCCTGAAAGACCGGACGGCGCAAAAGCGGGTTGC
CATGCGCCTGACGCGCATTGAATCGGGGCTTCTGGGCGACTGGAAAACACTCGGAGACGGCATCAGCGAACTGCGAATCG
ACCATGGCCCCGGATACCGGATTTACTTCACCCGGCGGGATCAACGGATCATCATCCTGTTGTGTGGCAGCGACAAGGGA
GATCAGGATCGGGCGATCAGACAGGCAAGGCAAATGGCCAAAGACCTTGATATCCCTCTGATGACATCTCCCCGTCCCCA
ACGACCTGGAACAACGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J9HHX2