Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 782668..783189 Replicon chromosome
Accession NZ_CP045085
Organism Staphylococcus pseudintermedius strain KCTC 43135

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag GAN20_RS03830 Protein ID WP_014613364.1
Coordinates 782668..782889 (-) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag GAN20_RS03835 Protein ID WP_014613363.1
Coordinates 782902..783189 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GAN20_RS03815 778250..778977 - 728 Protein_720 peptidase -
GAN20_RS12480 779945..780091 - 147 WP_014613367.1 hypothetical protein -
GAN20_RS03820 780177..782357 - 2181 WP_115807195.1 heavy metal translocating P-type ATPase -
GAN20_RS03825 782458..782664 - 207 WP_014613365.1 cation transporter -
GAN20_RS03830 782668..782889 - 222 WP_014613364.1 heavy-metal-associated domain-containing protein Toxin
GAN20_RS03835 782902..783189 - 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
GAN20_RS03840 783321..784811 + 1491 WP_014613362.1 cardiolipin synthase -
GAN20_RS03845 784832..785488 + 657 WP_014613361.1 HD domain-containing protein -
GAN20_RS03850 785542..786408 - 867 WP_014613360.1 membrane protein insertase YidC -
GAN20_RS03855 786497..787135 - 639 WP_014613359.1 thiamine phosphate synthase -
GAN20_RS03860 787125..787928 - 804 WP_014613358.1 hydroxyethylthiazole kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T141187 WP_014613364.1 NZ_CP045085:c782889-782668 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T141187 NZ_CP059851:2750558-2750851 [Sandaracinobacter sp. M6]
GTGATTGATGTCAAGCAGACGCGCATCTTTGCTGACTGGTTACGGGATTTGCGCGATGCGCGTGCACGGGACCGGATCGA
GCTTCGGCTCGCACGCATCGCTGTCGGGCATTTCGGTGACGTCAAACGCGTGGGCCGCAAACTGATCGAGGCCCGTGTGG
ACTATGGCCCCGGCTACCGTGTCTACCTGGTGCGGCGCGGACAGAAGCTGGTCGTCCTGCTCTGCGGCGGCGACAAATCC
AGCCAGCAGCGTGATATCGCGGCCGCATTGGCGATGATTGAGGAGTTGGATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT141187 WP_014613363.1 NZ_CP045085:c783189-782902 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT141187 NZ_CP059851:2750851-2751138 [Sandaracinobacter sp. M6]
ATGCCCCTGGACCTGCTGCCTTACGACGCCTCAGCCTGCCTCGTCGGTCGACCCGAAGAGCAGGCATCCCTGCTGTCCGA
TGCGCTGGCCACCAACGATCCGGATATTGTCGCCGGCGCGCTCGCGCTGATCGCCAGGGCGCAGGGTGTGAGCGCGATGG
CGCGCGAAACCGGCCTCAGCCGCGAAGCTATCTACAAGGCGACCCGCAAGGACGGCAATCCTCGGCTCTCCACGCTCATT
GCCATGCTGAAATCGGCCGGCATCAAACTCACCGCAACTGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References