Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 767750..768274 Replicon chromosome
Accession NZ_CP045085
Organism Staphylococcus pseudintermedius strain KCTC 43135

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag GAN20_RS03760 Protein ID WP_014613379.1
Coordinates 767750..768103 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag GAN20_RS03765 Protein ID WP_014613378.1
Coordinates 768104..768274 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GAN20_RS03740 765059..765829 - 771 WP_152162928.1 RNA polymerase sigma factor SigB -
GAN20_RS03745 765804..766283 - 480 WP_031253870.1 anti-sigma B factor RsbW -
GAN20_RS03750 766283..766609 - 327 WP_014613381.1 anti-sigma factor antagonist -
GAN20_RS03755 766688..767692 - 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
GAN20_RS03760 767750..768103 - 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GAN20_RS03765 768104..768274 - 171 WP_014613378.1 hypothetical protein Antitoxin
GAN20_RS03770 768359..769507 - 1149 WP_140239190.1 alanine racemase -
GAN20_RS03775 769541..769891 - 351 WP_014613376.1 holo-ACP synthase -
GAN20_RS03780 769888..770406 - 519 WP_014613375.1 PH domain-containing protein -
GAN20_RS03785 770390..771919 - 1530 WP_014613374.1 PH domain-containing protein -
GAN20_RS03790 771912..772385 - 474 WP_014613373.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T141186 WP_014613379.1 NZ_CP045085:c768103-767750 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T141186 NZ_CP059851:2118984-2119265 [Sandaracinobacter sp. M6]
ATGCGGACGATTGAACGCACCACGACGTTCAAGCGAGACTGGAAACGCGAACTCAGGGGCCGTCACGCCGCCACACCCGC
CCGCGATGTCACCGGGCTCCTCACGCTGCTCGTCCACGACCAGCCGCTCGAACCCCGCCACCGTGATCATGCCCTCACCG
GCAACTGGAAGGACCATCGGGACTGTCATGTCAAACCCGACCTGGTGCTGATCTACCGAAAGCCGGACCCCGAAACCCTG
CAACTGGTTCGATTGGGCTCCCACGCCGAACTCGGCTGGTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT141186 WP_014613378.1 NZ_CP045085:c768274-768104 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT141186 NZ_CP059851:2118734-2118997 [Sandaracinobacter sp. M6]
ATGTCCAGCAATGCCCTCGTCCAGACCCGCATCAACGAAACCGTCAAGCAGCAGGCTGCCACCGTCCTTGCCACCATGGG
CCTCACCGTGTCCGATGCCGTCCGCATGATGCTCACCCGCGTCGCGCACGACAAGGCGCTGCCCTTCGATCCGCTCGTGC
CGAACGCCGAAACCATCGCCGCCATGGAACAGGCCCGCGCAGGCGGCCTGCCGCGTTTCAAATCGGTCGATGCGCTGATG
GCCGATCTCGATGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References