Detailed information of TA system TA141170

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3983282..3983961
• Replicon: chromosome
• Accession: NZ_CP045064
• Organism: Enterobacter roggenkampii strain WCHER090065

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A8B3US07
• Locus tag: GIX98_RS19170
• Protein ID: WP_007777710.1
• Coordinates: 3983282..3983623 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B3USS8
• Locus tag: GIX98_RS19175
• Protein ID: WP_007777712.1
• Coordinates: 3983644..3983961 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIX98_RS19150	3979225..3980193	-	969	WP_000654812.1	IS5-like element IS903B family transposase	-
GIX98_RS19155	3980340..3981443	+	1104	WP_021242883.1	glutamate 5-kinase	-
GIX98_RS19160	3981455..3982708	+	1254	WP_058654073.1	glutamate-5-semialdehyde dehydrogenase	-
GIX98_RS19170	3983282..3983623	-	342	WP_007777710.1	TA system toxin CbtA family protein	Toxin
GIX98_RS19175	3983644..3983961	-	318	WP_007777712.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GIX98_RS19180	3983982..3984203	-	222	WP_007777714.1	DUF987 domain-containing protein	-
GIX98_RS19185	3984212..3984688	-	477	WP_000811693.1	RadC family protein	-
GIX98_RS19190	3984704..3985162	-	459	WP_126287411.1	antirestriction protein	-
GIX98_RS19195	3985260..3985499	-	240	WP_000194654.1	DUF905 domain-containing protein	-
GIX98_RS19200	3985576..3986043	-	468	WP_001385283.1	hypothetical protein	-
GIX98_RS19205	3986066..3986509	-	444	WP_126287412.1	hypothetical protein	-
GIX98_RS19210	3986509..3986745	-	237	WP_001144031.1	hypothetical protein	-
GIX98_RS19215	3986786..3987487	-	702	WP_000189411.1	WYL domain-containing protein	-
GIX98_RS19220	3987704..3988525	-	822	WP_000197387.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3976077..4018757	42680
	-	flank	IS/Tn	-	-	3979225..3980193	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12934.88 Da
• Isoelectric Point: 8.0308

>T141170 WP_007777710.1 NZ_CP045064:c3983623-3983282 [Enterobacter roggenkampii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLQAVDILRARQATGLLQQSRSNVVR

Nucleotide

• Length: 342 bp

>T141170 NZ_CP059843:c2274189-2273896 [Cobetia sp. 4B]
ATGGCGGTGGAGTTCAGGGATGTGTGGCTCGAGAGCTTCTATGAGCATGATGTTGGCCACCGCAAGATACCTGCGGCGCT
CTCCAGTTCGCTCTATCGCAAGTTGCAGATTCTCGATGCCGCAAGTCAGGAGTCTGATCTGAGGATTCCACCGGGCAATC
GATTCGAGCATCTGCAGGGCAATCTCGCAGGTTATTGCTCGATTCGTGTCAACAAGCAGTATCGACTCATCTTTCGATGG
GAGGCAGGGATTGCCCATCAAACATATCTAGACCCGCATGCTTACAAGGGCTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12005.67 Da
• Isoelectric Point: 7.3723

>AT141170 WP_007777712.1 NZ_CP045064:c3983961-3983644 [Enterobacter roggenkampii]
MTHITWGLQRNITPRLGARLVQEGNRLHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVIMYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT141170 NZ_CP059843:c2273881-2273552 [Cobetia sp. 4B]
ATGAGAAACACCCAGCGGCGCCCACTGACGGTAGGAGAGATGTTGACGGCAGAGTTTCTGGAGCCGATGGACATCGACAT
CCGCACCCTGGCCGAGGCGATGGGAGTGCACAGAAATACCCTGAGCCGTATCGTGCATGACAAGGGCACCTTGACGGCCC
CGATGGCCATCAAGCTGGCGGCTGCGTTAGGCAATACGCCGGAGTTCTGGCTCAACATCCAGCATGCCGTGGAGCTTTGG
GATGTGCGTCATCGTGCCTATGCCCAGGATGCCAAGGGCGTACGTCGGATAACGCCGCAAGTTGATGCCACCGAGCTTGC
CTCGCACTGA

Structures

Toxin

• AlphaFold DB: A0A8B3US07

Antitoxin

• AlphaFold DB: A0A8B3USS8