Detailed information of TA system TA141142

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 249802..250451
• Replicon: chromosome
• Accession: NZ_CP045051
• Organism: Acinetobacter johnsonii strain AJ312

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: GBN67_RS01265
• Protein ID: WP_234963406.1
• Coordinates: 250065..250451 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: N9CU98
• Locus tag: GBN67_RS01260
• Protein ID: WP_004977895.1
• Coordinates: 249802..250059 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBN67_RS01240 (GBN67_01245)	245420..245785	+	366	WP_004691290.1	50S ribosomal protein L17	-
GBN67_RS01245 (GBN67_01250)	245982..247472	+	1491	WP_234963403.1	NAD(P)/FAD-dependent oxidoreductase	-
GBN67_RS01250 (GBN67_01255)	247610..248401	+	792	WP_234963404.1	alpha/beta hydrolase	-
GBN67_RS01255 (GBN67_01260)	248437..249609	-	1173	WP_234963405.1	acyl-CoA dehydrogenase family protein	-
GBN67_RS01260 (GBN67_01265)	249802..250059	+	258	WP_004977895.1	succinate dehydrogenase assembly factor 2	Antitoxin
GBN67_RS01265 (GBN67_01270)	250065..250451	+	387	WP_234963406.1	hypothetical protein	Toxin
GBN67_RS01270	250972..252009	+	1038	WP_234963407.1	hypothetical protein	-
GBN67_RS01275 (GBN67_01280)	252081..252653	+	573	WP_234963408.1	rhombosortase	-
GBN67_RS01280 (GBN67_01285)	252836..255118	+	2283	WP_234963409.1	TRAP transporter large permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-127)

Antitoxin

• Flavinator of succinate dehydrogenase (10-82)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 15330.34 Da
• Isoelectric Point: 10.4419

>T141142 WP_234963406.1 NZ_CP045051:250065-250451 [Acinetobacter johnsonii]
MEHRHFQLKPSRLAYVFQLSIYAVLMLVLYQLLPLGLWLIALIAGFVLYLFFLRGPQHFQLQHLDGRDWSLSLQKQTNVQ
RVQISHIVDHRAYIVIYFQHFRAKPMLVWCDQLPITQWKALKILTKMQ

Nucleotide

• Length: 387 bp

>T141142 NZ_CP059837:c75642-75364 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10181.71 Da
• Isoelectric Point: 4.7119

>AT141142 WP_004977895.1 NZ_CP045051:249802-250059 [Acinetobacter johnsonii]
MTEEMSLEERKVIYRARRGLKEIDVYFDPYVKNYYLQADPAEKAMFAALVEQEDPDLLDWFMEVSEPPRPEMKTLIQKLK
HYVHG

Nucleotide

• Length: 258 bp

>AT141142 NZ_CP059837:c75911-75642 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: N9CU98