Detailed information of TA system TA141141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 6001..6572
• Replicon: plasmid pLTR1318
• Accession: NZ_CP045050
• Organism: Limosilactobacillus reuteri strain reuteri

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7XA07
• Locus tag: F9294_RS10470
• Protein ID: WP_012621080.1
• Coordinates: 6240..6572 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7XA06
• Locus tag: F9294_RS10465
• Protein ID: WP_012621079.1
• Coordinates: 6001..6246 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9294_RS10425	1360..1641	+	282	WP_152111329.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
F9294_RS10430	1688..1873	+	186	WP_152111331.1	hypothetical protein	-
F9294_RS10435	1870..2550	+	681	WP_152111333.1	zeta toxin family protein	-
F9294_RS10440	2540..2818	-	279	WP_041823115.1	hypothetical protein	-
F9294_RS10445	2841..3068	-	228	WP_191986930.1	hypothetical protein	-
F9294_RS10450	3321..5384	+	2064	WP_152111337.1	MobA/MobL family protein	-
F9294_RS10460	5775..5864	-	90	WP_021353390.1	putative holin-like toxin	-
F9294_RS10465	6001..6246	+	246	WP_012621079.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F9294_RS10470	6240..6572	+	333	WP_012621080.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F9294_RS10475	7496..7999	+	504	WP_152111339.1	SMI1/KNR4 family protein	-
F9294_RS10480	8291..9034	+	744	WP_152111341.1	nicotinamide mononucleotide transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20412	20412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12564.66 Da
• Isoelectric Point: 10.0372

>T141141 WP_012621080.1 NZ_CP045050:6240-6572 [Limosilactobacillus reuteri]
MVGIKQGSIIKINLDPKQGHEQKGYRPYICLSHSIVTKYSNLAIFAPISNTKRKYPFYVPLKDTETTGKVLLDQLVTIDF
NARDYRYIENVSDKLLSSLLARVKVLFEKG

Nucleotide

• Length: 333 bp

>T141141 NZ_CP059837:39723-40139 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9250.54 Da
• Isoelectric Point: 5.5332

>AT141141 WP_012621079.1 NZ_CP045050:6001-6246 [Limosilactobacillus reuteri]
MQLTVNKWGNSSAIRLPKQLVQQLQLHTNDILDYEVSGNKIILKKATTVPELTVDDLFKDYHGEPVSVTPFIFESVGHEQ
W

Nucleotide

• Length: 246 bp

>AT141141 NZ_CP059837:39496-39726 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: B7XA07

Antitoxin

• AlphaFold DB: B7XA06