Detailed information of TA system TA141137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1572324..1573461
• Replicon: chromosome
• Accession: NZ_CP045045
• Organism: Enterococcus faecalis strain JY32

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: F9293_RS07515
• Protein ID: WP_002401483.1
• Coordinates: 1572598..1573461 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: F9293_RS07510
• Protein ID: WP_000301765.1
• Coordinates: 1572324..1572596 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9293_RS07475	1567620..1569296	+	1677	WP_002346508.1	type IA DNA topoisomerase	-
F9293_RS07480	1569422..1569586	+	165	WP_002935077.1	peptide-binding protein	-
F9293_RS07485	1569607..1569702	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
F9293_RS07490	1569827..1570564	+	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
F9293_RS07495	1570710..1570994	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
F9293_RS07500	1571097..1571993	+	897	WP_002387620.1	ParA family protein	-
F9293_RS07505	1572098..1572307	+	210	WP_000527317.1	peptide-binding protein	-
F9293_RS07510	1572324..1572596	+	273	WP_000301765.1	antitoxin	Antitoxin
F9293_RS07515	1572598..1573461	+	864	WP_002401483.1	zeta toxin family protein	Toxin
F9293_RS07520	1573902..1574219	+	318	WP_002333463.1	hypothetical protein	-
F9293_RS07525	1574239..1574460	+	222	Protein_1488	DnaJ domain-containing protein	-
F9293_RS07530	1574537..1575217	+	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
F9293_RS07535	1575273..1575704	+	432	Protein_1490	2-dehydropantoate 2-reductase	-
F9293_RS07540	1575980..1576831	+	852	WP_002357480.1	ribosome biogenesis GTPase YlqF	-
F9293_RS07545	1576833..1577600	+	768	WP_002357481.1	ribonuclease HII	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	str / cat / erm(B)	-	1562417..1575217	12800

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T141137 WP_002401483.1 NZ_CP045045:1572598-1573461 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T141137 NZ_CP059835:c5011902-5011795 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT141137 WP_000301765.1 NZ_CP045045:1572324-1572596 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT141137 NZ_CP059835:5011950-5012016 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3