Detailed information of TA system TA141136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1281630..1282323
• Replicon: chromosome
• Accession: NZ_CP045045
• Organism: Enterococcus faecalis strain JY32

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: S4BLV4
• Locus tag: F9293_RS05985
• Protein ID: WP_002378467.1
• Coordinates: 1281630..1281974 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: F9293_RS05990
• Protein ID: WP_002364355.1
• Coordinates: 1281991..1282323 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9293_RS05955	1276904..1277872	+	969	WP_002364362.1	Flp pilus assembly complex ATPase component TadA	-
F9293_RS05960	1277829..1278875	+	1047	WP_002369171.1	type II secretion system F family protein	-
F9293_RS05965	1278875..1279150	+	276	WP_002356991.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
F9293_RS05970	1279147..1279677	+	531	WP_002378470.1	type II secretion system GspH family protein	-
F9293_RS05975	1279618..1280766	-	1149	WP_002378469.1	site-specific integrase	-
F9293_RS05980	1280866..1281594	-	729	WP_002378468.1	potassium channel family protein	-
F9293_RS05985	1281630..1281974	-	345	WP_002378467.1	ImmA/IrrE family metallo-endopeptidase	Toxin
F9293_RS05990	1281991..1282323	-	333	WP_002364355.1	helix-turn-helix domain-containing protein	Antitoxin
F9293_RS05995	1282635..1282811	+	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
F9293_RS06000	1282822..1283133	+	312	WP_002381719.1	hypothetical protein	-
F9293_RS06005	1283172..1283897	+	726	WP_002378466.1	phage regulatory protein	-
F9293_RS06010	1283923..1284111	-	189	WP_002357001.1	YegP family protein	-
F9293_RS06015	1284166..1284375	+	210	WP_002378465.1	hypothetical protein	-
F9293_RS06020	1284412..1284748	+	337	Protein_1188	hypothetical protein	-
F9293_RS06025	1285033..1285587	-	555	WP_002357006.1	hypothetical protein	-
F9293_RS06030	1285807..1286124	+	318	WP_002357007.1	hypothetical protein	-
F9293_RS06035	1286117..1286851	+	735	WP_002378463.1	ERF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1279174..1318661	39487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13712.62 Da
• Isoelectric Point: 5.5523

>T141136 WP_002378467.1 NZ_CP045045:c1281974-1281630 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYIELYKIPSFRNKMEAEADY
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T141136 NZ_CP059835:4256380-4256778 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12691.16 Da
• Isoelectric Point: 4.6307

>AT141136 WP_002364355.1 NZ_CP045045:c1282323-1281991 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT141136 NZ_CP059835:4256153-4256380 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: S4BLV4