Detailed information of TA system TA141130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 10069..10670
• Replicon: plasmid unnamed1
• Accession: NZ_CP045036
• Organism: Liquorilactobacillus mali strain LM596

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: LM596_RS12215
• Protein ID: WP_001748110.1
• Coordinates: 10326..10670 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LM596_RS12210
• Protein ID: WP_003689111.1
• Coordinates: 10069..10332 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM596_RS12190	6537..7502	+	966	WP_003691075.1	AEC family transporter	-
LM596_RS12195	7623..8567	-	945	WP_152134800.1	IS3 family transposase	-
LM596_RS12200	8519..9052	-	534	WP_002331365.1	helix-turn-helix domain-containing protein	-
LM596_RS12205	9396..9983	-	588	WP_010078383.1	site-specific integrase	-
LM596_RS12210	10069..10332	+	264	WP_003689111.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LM596_RS12215	10326..10670	+	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LM596_RS12220	10815..11741	-	927	WP_003689112.1	abortive infection family protein	-
LM596_RS12225	11762..14371	-	2610	WP_003689113.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60578	60578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T141130 WP_001748110.1 NZ_CP045036:10326-10670 [Liquorilactobacillus mali]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T141130 NZ_CP059835:c2739258-2739028 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.50 Da
• Isoelectric Point: 5.7235

>AT141130 WP_003689111.1 NZ_CP045036:10069-10332 [Liquorilactobacillus mali]
MEPIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EDNELQW

Nucleotide

• Length: 264 bp

>AT141130 NZ_CP059835:c2739031-2738672 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCATATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3