Detailed information of TA system TA141129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 2064066..2064667
• Replicon: chromosome
• Accession: NZ_CP045035
• Organism: Liquorilactobacillus mali strain LM596

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: J0KWT2
• Locus tag: LM596_RS10145
• Protein ID: WP_003691102.1
• Coordinates: 2064323..2064667 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LM596_RS10140
• Protein ID: WP_003691100.1
• Coordinates: 2064066..2064329 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM596_RS10100	2059788..2060750	-	963	WP_003691088.1	2-hydroxyacid dehydrogenase family protein	-
LM596_RS10105	2060850..2061248	+	399	WP_003691089.1	helix-turn-helix transcriptional regulator	-
LM596_RS10110	2061255..2061728	-	474	WP_003691091.1	hypothetical protein	-
LM596_RS10115	2061950..2062456	-	507	WP_034542111.1	hypothetical protein	-
LM596_RS10120	2062446..2062727	-	282	WP_003691096.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LM596_RS10125	2062886..2063293	-	408	WP_003691098.1	hypothetical protein	-
LM596_RS10135	2063688..2063999	-	312	WP_152134775.1	hypothetical protein	-
LM596_RS10140	2064066..2064329	+	264	WP_003691100.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LM596_RS10145	2064323..2064667	+	345	WP_003691102.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LM596_RS10150	2064778..2065311	+	534	WP_152134776.1	transposase	-
LM596_RS10155	2065308..2066207	+	900	WP_152134777.1	IS3 family transposase	-
LM596_RS10160	2066901..2068139	-	1239	WP_003691574.1	2-oxo acid dehydrogenase subunit E2	-
LM596_RS10165	2068153..2069184	-	1032	WP_003691575.1	alpha-ketoacid dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-102)

Antitoxin

• SpoVT-AbrB domain (12-55)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12983.67 Da
• Isoelectric Point: 4.5326

>T141129 WP_003691102.1 NZ_CP045035:2064323-2064667 [Liquorilactobacillus mali]
MVSQGEIFYVDFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHPLTSSQTVYGKVLLDQTIALDLR
ARHVTDEAIVDHVSREELEEIISLYKLLFSIDDE

Nucleotide

• Length: 345 bp

>T141129 NZ_CP059835:2414325-2414789 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGTGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10123.48 Da
• Isoelectric Point: 6.4708

>AT141129 WP_003691100.1 NZ_CP045035:2064066-2064329 [Liquorilactobacillus mali]
MEIIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDLTITIEKGSIVLTPVKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT141129 NZ_CP059835:2413990-2414325 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: J0KWT2