Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1746616..1747199 Replicon chromosome
Accession NZ_CP045033
Organism Lactobacillus kefiranofaciens subsp. kefiranofaciens strain LKK75

Toxin (Protein)


Gene name mazF Uniprot ID A0A656Y774
Locus tag LKK75_RS08710 Protein ID WP_025084310.1
Coordinates 1746861..1747199 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A656Y737
Locus tag LKK75_RS08705 Protein ID WP_013851202.1
Coordinates 1746616..1746867 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKK75_RS08670 1742324..1742605 - 282 WP_013851193.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
LKK75_RS08675 1742612..1742899 - 288 WP_025084314.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LKK75_RS08680 1743132..1744208 + 1077 WP_013851195.1 Fic family protein -
LKK75_RS08685 1744402..1744647 + 246 WP_013851196.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LKK75_RS08690 1744650..1744979 + 330 WP_013851197.1 type II toxin-antitoxin system RelE/ParE family toxin -
LKK75_RS08695 1745137..1745727 + 591 WP_025084312.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
LKK75_RS08700 1745758..1745958 + 201 Protein_1680 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LKK75_RS11610 1746284..1746424 + 141 WP_013851201.1 hypothetical protein -
LKK75_RS08705 1746616..1746867 + 252 WP_013851202.1 hypothetical protein Antitoxin
LKK75_RS08710 1746861..1747199 + 339 WP_025084310.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LKK75_RS08715 1747698..1748732 + 1035 WP_025084309.1 IS30 family transposase -
LKK75_RS08720 1748888..1750066 + 1179 Protein_1685 IS256 family transposase -
LKK75_RS08725 1750261..1751538 - 1278 WP_013851207.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13056.26 Da        Isoelectric Point: 9.8423

>T141127 WP_025084310.1 NZ_CP045033:1746861-1747199 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MVIKQGSVLLMDLSPKQGHEQSGKRPYLYLSYQGVEKYAHIAIFAPISTTKRRYPLYRNLPDSCKTKGVVMLDQLVTVDY
HNRKHQFLEQLPDDYMEKLLKIVKVVFQKDPS

Download         Length: 339 bp

>T141127 NZ_CP059835:c2061734-2061384 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9503.84 Da        Isoelectric Point: 9.6573

>AT141127 WP_013851202.1 NZ_CP045033:1746616-1746867 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MITATISKWGNSKGIRLNQAVLQALNAKTGDHLNFKIEDNRVIITKKEPKHELTFEELFKDYKGGSFDTKISLPDERVGN
EKW

Download         Length: 252 bp

>AT141127 NZ_CP059835:c2061979-2061728 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656Y774

Antitoxin

Source ID Structure
AlphaFold DB A0A656Y737

References