Detailed information of TA system TA141125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2312934..2313912
• Replicon: chromosome
• Accession: NZ_CP045030
• Organism: Bacillus thuringiensis strain JW-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: F9800_RS17895
• Protein ID: WP_000624969.1
• Coordinates: 2312934..2313671 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B7IX39
• Locus tag: F9800_RS17900
• Protein ID: WP_000237817.1
• Coordinates: 2313784..2313912 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9800_RS17865	2308525..2308932	+	408	WP_000072495.1	VOC family protein	-
F9800_RS17870	2309277..2310059	+	783	WP_000381785.1	class I SAM-dependent methyltransferase	-
F9800_RS17875	2310213..2311058	-	846	Protein_2264	alpha-keto acid decarboxylase family protein	-
F9800_RS17880	2311034..2311202	-	169	Protein_2265	hypothetical protein	-
F9800_RS17885	2311310..2311792	+	483	WP_000191905.1	MarR family winged helix-turn-helix transcriptional regulator	-
F9800_RS17890	2311958..2312695	+	738	WP_000594159.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
F9800_RS17895	2312934..2313671	+	738	WP_000624969.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F9800_RS17900	2313784..2313912	+	129	WP_000237817.1	hypothetical protein	Antitoxin
F9800_RS17905	2313985..2314161	+	177	WP_000852629.1	stage II sporulation protein SB	-
F9800_RS17910	2314180..2314569	-	390	WP_000712655.1	YxeA family protein	-
F9800_RS17915	2314781..2316250	+	1470	WP_000287541.1	beta-Ala-His dipeptidase	-
F9800_RS17920	2316563..2317372	+	810	WP_000678376.1	C39 family peptidase	-
F9800_RS17925	2317398..2318003	+	606	WP_000527414.1	hypothetical protein	-
F9800_RS17930	2318144..2318395	-	252	WP_000821942.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (11-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28383.13 Da
• Isoelectric Point: 8.2998

>T141125 WP_000624969.1 NZ_CP045030:2312934-2313671 [Bacillus thuringiensis]
MISNIRIGLFVLAIFFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T141125 NZ_CP059835:1463586-1464128 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4981.37 Da
• Isoelectric Point: 3.9150

>AT141125 WP_000237817.1 NZ_CP045030:2313784-2313912 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT141125 NZ_CP059835:1463291-1463602 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: B7IX39