141124

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 231068..231710 Replicon chromosome
Accession NZ_CP045030
Organism Bacillus thuringiensis strain JW-1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag F9800_RS07155 Protein ID WP_000635965.1
Coordinates 231360..231710 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag F9800_RS07150 Protein ID WP_000004570.1
Coordinates 231068..231355 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9800_RS07125 226385..227347 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
F9800_RS07130 227340..227912 - 573 WP_000908528.1 rhomboid family intramembrane serine protease -
F9800_RS07135 228006..228365 + 360 WP_000583420.1 holo-ACP synthase -
F9800_RS07140 228522..229472 + 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
F9800_RS07145 229590..230759 + 1170 WP_000390594.1 alanine racemase -
F9800_RS07150 231068..231355 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
F9800_RS07155 231360..231710 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
F9800_RS07160 231778..233946 + 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
F9800_RS07165 234004..234120 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
F9800_RS07170 234316..234774 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T141124 WP_000635965.1 NZ_CP045030:231360-231710 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T141124 NZ_CP059835:1429615-1429833 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT141124 WP_000004570.1 NZ_CP045030:231068-231355 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT141124 NZ_CP059835:1429215-1429589 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References