Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5135203..5135828 Replicon chromosome
Accession NZ_CP045015
Organism Klebsiella pneumoniae subsp. pneumoniae strain BK13048

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag F9865_RS25350 Protein ID WP_040182550.1
Coordinates 5135203..5135586 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag F9865_RS25355 Protein ID WP_004150355.1
Coordinates 5135586..5135828 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9865_RS25335 5132569..5133471 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
F9865_RS25340 5133468..5134103 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
F9865_RS25345 5134100..5135029 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
F9865_RS25350 5135203..5135586 - 384 WP_040182550.1 type II toxin-antitoxin system VapC family toxin Toxin
F9865_RS25355 5135586..5135828 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
F9865_RS25360 5136033..5136950 + 918 WP_023302328.1 alpha/beta hydrolase -
F9865_RS25365 5136964..5137905 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
F9865_RS25370 5137950..5138387 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
F9865_RS25375 5138384..5139244 - 861 WP_004146232.1 virulence factor BrkB family protein -
F9865_RS25380 5139238..5139837 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14376.67 Da        Isoelectric Point: 9.5352

>T141118 WP_040182550.1 NZ_CP045015:c5135586-5135203 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T141118 NZ_CP059823:478694-479035 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTAAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT141118 WP_004150355.1 NZ_CP045015:c5135828-5135586 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT141118 NZ_CP059823:478464-478694 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References