Detailed information of TA system TA141115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4571313..4572123
• Replicon: chromosome
• Accession: NZ_CP045015
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain BK13048

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: F9865_RS22700
• Protein ID: WP_014908042.1
• Coordinates: 4571313..4571846 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: F9865_RS22705
• Protein ID: WP_023343051.1
• Coordinates: 4571857..4572123 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9865_RS22695	4570144..4571265	+	1122	WP_012737335.1	cupin domain-containing protein	-
F9865_RS22700	4571313..4571846	-	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
F9865_RS22705	4571857..4572123	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
F9865_RS22710	4572226..4573659	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
F9865_RS22715	4573649..4574332	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
F9865_RS22720	4574505..4575890	+	1386	WP_040182471.1	efflux transporter outer membrane subunit	-
F9865_RS22725	4575908..4576252	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T141115 WP_014908042.1 NZ_CP045015:c4571846-4571313 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T141115 NZ_CP059818:22964-23314 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT141115 WP_023343051.1 NZ_CP045015:c4572123-4571857 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT141115 NZ_CP059818:22707-22970 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A332NV98