Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2556351..2557939 Replicon chromosome
Accession NZ_CP045015
Organism Klebsiella pneumoniae subsp. pneumoniae strain BK13048

Toxin (Protein)


Gene name hipA Uniprot ID A0A486S9S0
Locus tag F9865_RS12745 Protein ID WP_023301940.1
Coordinates 2556351..2557673 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag F9865_RS12750 Protein ID WP_002904790.1
Coordinates 2557673..2557939 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9865_RS12725 2551407..2553908 + 2502 WP_040182042.1 fimbrial biogenesis outer membrane usher protein -
F9865_RS12730 2553896..2554900 + 1005 WP_009308876.1 type 1 fimbrial protein -
F9865_RS12740 2555042..2556334 + 1293 WP_016946381.1 glycoside hydrolase family 10 protein -
F9865_RS12745 2556351..2557673 - 1323 WP_023301940.1 type II toxin-antitoxin system HipA family toxin Toxin
F9865_RS12750 2557673..2557939 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
F9865_RS12755 2558205..2558531 - 327 WP_004143476.1 hydroxyisourate hydrolase -
F9865_RS12760 2558528..2559028 - 501 WP_040182039.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
F9865_RS12765 2559006..2560385 - 1380 WP_004176204.1 purine permease -
F9865_RS12770 2560731..2561885 + 1155 WP_040182036.1 FAD-dependent urate hydroxylase HpxO -
F9865_RS12775 2561866..2562789 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49185.49 Da        Isoelectric Point: 7.5358

>T141113 WP_023301940.1 NZ_CP045015:c2557673-2556351 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T141113 NZ_CP059817:18472-18729 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT141113 WP_002904790.1 NZ_CP045015:c2557939-2557673 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT141113 NZ_CP059817:18210-18479 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486S9S0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References