Detailed information of TA system TA141101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 184842..185950
• Replicon: plasmid pI
• Accession: NZ_CP045013
• Organism: Enterococcus faecium strain LAC7.2

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A0M4MHJ8
• Locus tag: F6447_RS14045
• Protein ID: WP_002303392.1
• Coordinates: 184842..185711 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: F6447_RS14050
• Protein ID: WP_002303393.1
• Coordinates: 185726..185950 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6447_RS14010	180273..181010	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
F6447_RS14015	181135..181218	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
F6447_RS14020	181267..181350	-	84	Protein_180	peptide-binding protein	-
F6447_RS14025	181760..182554	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
F6447_RS14030	182647..183189	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
F6447_RS14035	183186..184094	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
F6447_RS14040	184127..184861	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
F6447_RS14045	184842..185711	-	870	WP_002303392.1	nucleotidyltransferase domain-containing protein	Toxin
F6447_RS14050	185726..185950	-	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
F6447_RS14055	186093..186914	-	822	Protein_187	recombinase zinc beta ribbon domain-containing protein	-
F6447_RS14060	187602..188405	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
F6447_RS14065	188459..189913	-	1455	WP_167746207.1	ABC-F type ribosomal protection protein Lsa(E)	-
F6447_RS14070	190387..190950	-	564	Protein_190	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32807.38 Da
• Isoelectric Point: 4.7269

>T141101 WP_002303392.1 NZ_CP045013:c185711-184842 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T141101 NZ_CP059793:1478691-1478957 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT141101 WP_002303393.1 NZ_CP045013:c185950-185726 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT141101 NZ_CP059793:1478428-1478691 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0M4MHJ8

Antitoxin

• AlphaFold DB: A0A1M5SHM7