141100

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 536154..536725 Replicon chromosome
Accession NZ_CP045012
Organism Enterococcus faecium strain LAC7.2

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag F6447_RS02635 Protein ID WP_002286801.1
Coordinates 536384..536725 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag F6447_RS02630 Protein ID WP_002323011.1
Coordinates 536154..536384 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6447_RS02595 531410..532741 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
F6447_RS02600 532763..533389 + 627 WP_002286816.1 cysteine hydrolase -
F6447_RS02605 533572..534153 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
F6447_RS02610 534762..535109 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
F6447_RS02615 535085..535324 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
F6447_RS02620 535529..535867 - 339 WP_002286804.1 hypothetical protein -
F6447_RS02630 536154..536384 + 231 WP_002323011.1 hypothetical protein Antitoxin
F6447_RS02635 536384..536725 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F6447_RS02640 537575..537760 + 186 WP_002304207.1 hypothetical protein -
F6447_RS02645 538265..538459 + 195 WP_002297028.1 hypothetical protein -
F6447_RS02650 538650..538899 + 250 Protein_509 transposase -
F6447_RS02660 539143..539973 - 831 WP_002286789.1 manganese catalase family protein -
F6447_RS02665 540181..541515 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T141100 WP_002286801.1 NZ_CP045012:536384-536725 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T141100 NZ_CP059787:7948-8205 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT141100 WP_002323011.1 NZ_CP045012:536154-536384 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT141100 NZ_CP059787:7686-7955 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References