Detailed information of TA system TA141098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 29462..29970
• Replicon: plasmid pZF2-cfr
• Accession: NZ_CP045009
• Organism: Proteus terrae subsp. cibarius strain ZF2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: F9282_RS19975
• Protein ID: WP_050878415.1
• Coordinates: 29695..29970 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F9282_RS19970
• Protein ID: WP_050878414.1
• Coordinates: 29462..29686 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9282_RS19935	24963..25172	-	210	WP_050878402.1	hypothetical protein	-
F9282_RS19940	25176..25460	-	285	WP_050878404.1	hypothetical protein	-
F9282_RS19945	25514..26293	-	780	WP_050878406.1	hypothetical protein	-
F9282_RS19950	26330..26572	-	243	WP_156734530.1	hypothetical protein	-
F9282_RS19955	26650..26985	-	336	WP_050878410.1	hypothetical protein	-
F9282_RS19960	27549..27929	-	381	WP_050878412.1	hypothetical protein	-
F9282_RS19965	28027..29250	-	1224	WP_159364217.1	DUF3560 domain-containing protein	-
F9282_RS19970	29462..29686	+	225	WP_050878414.1	hypothetical protein	Antitoxin
F9282_RS19975	29695..29970	+	276	WP_050878415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F9282_RS19980	30674..30847	-	174	WP_154393749.1	hypothetical protein	-
F9282_RS19985	31517..32347	+	831	WP_050878417.1	replication initiation protein	-
F9282_RS19990	32357..33184	+	828	WP_050878418.1	GIY-YIG nuclease family protein	-
F9282_RS19995	33281..33616	+	336	WP_050878420.1	hypothetical protein	-
F9282_RS20000	33724..34017	+	294	WP_050878422.1	sigma-70 family RNA polymerase sigma factor	-
F9282_RS20005	34210..34518	+	309	WP_050878424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cfr	-	1..59167	59167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (5-70)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11101.99 Da
• Isoelectric Point: 10.1375

>T141098 WP_050878415.1 NZ_CP045009:29695-29970 [Proteus terrae subsp. cibarius]
MDHYRIEYTKRFQKEIKKIDKYHQKIIQNYIEKHIDNSINPRLIGKILKGDKAEFIRYRIGDYRLIATIEDDKLLILALS
IGHRKDIYKHF

Nucleotide

• Length: 276 bp

>T141098 NZ_CP059784:183541-183807 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8490.46 Da
• Isoelectric Point: 4.2696

>AT141098 WP_050878414.1 NZ_CP045009:29462-29686 [Proteus terrae subsp. cibarius]
MRKNSTKAIRLNDDELTLFESYANAKGLTFSELCKSAVIEKIEDEIDLKLAEESYNEYLNDNETLSHKALFDSL

Nucleotide

• Length: 225 bp

>AT141098 NZ_CP059784:183278-183541 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA