Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 3169934..3170912 Replicon chromosome
Accession NZ_CP044978
Organism Bacillus thuringiensis strain BT62

Toxin (Protein)


Gene name spoIISA Uniprot ID W8Y388
Locus tag DDE73_RS16455 Protein ID WP_000624977.1
Coordinates 3169934..3170671 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID M1PKC2
Locus tag DDE73_RS16460 Protein ID WP_000237819.1
Coordinates 3170784..3170912 (+) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDE73_RS16430 3165546..3165954 + 409 Protein_3091 VOC family protein -
DDE73_RS16435 3165978..3166358 + 381 WP_000497668.1 methyltransferase -
DDE73_RS16440 3166516..3168201 - 1686 WP_193217111.1 alpha-keto acid decarboxylase family protein -
DDE73_RS16445 3168309..3168791 + 483 WP_000191889.1 MarR family winged helix-turn-helix transcriptional regulator -
DDE73_RS16450 3168958..3169695 + 738 WP_000594152.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
DDE73_RS16455 3169934..3170671 + 738 WP_000624977.1 stage II sporulation protein SA Toxin
DDE73_RS16460 3170784..3170912 + 129 WP_000237819.1 hypothetical protein Antitoxin
DDE73_RS16465 3170985..3171161 + 177 WP_000852629.1 stage II sporulation protein SB -
DDE73_RS16470 3171181..3171570 - 390 WP_000713862.1 YxeA family protein -
DDE73_RS16475 3171782..3173251 + 1470 WP_016124565.1 beta-Ala-His dipeptidase -
DDE73_RS16480 3173497..3174306 + 810 WP_103622414.1 C39 family peptidase -
DDE73_RS16485 3174332..3174934 + 603 WP_000517271.1 hypothetical protein -
DDE73_RS16490 3175178..3175429 - 252 WP_000827560.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28307.03 Da        Isoelectric Point: 8.2998

>T141082 WP_000624977.1 NZ_CP044978:3169934-3170671 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T141082 NZ_CP059755:460372-460713 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATACGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4953.36 Da        Isoelectric Point: 3.9798

>AT141082 WP_000237819.1 NZ_CP044978:3170784-3170912 [Bacillus thuringiensis]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Download         Length: 129 bp

>AT141082 NZ_CP059755:460142-460372 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W8Y388

Antitoxin

Source ID Structure
AlphaFold DB M1PKC2

References